Immunoassay approach for diagnosis of exposure to pyrrolizidine alkaloids

Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews
Na LiGe Lin

Abstract

Numerous pyrrolizidine alkaloid (PA) poisoning cases have been documented worldwide. Protein covalent binding with reactive metabolites generated from metabolic activation of PAs to form pyrrole-protein adducts is suggested to be a primary mechanism of PA-induced toxicities. The present study aimed to develop antibodies for diagnosis of PA exposure. Polyclonal antibodies were raised in rabbits and proven to specifically recognize pyrrole-protein adducts regardless of amino acid residues modified by the reactive metabolites of PAs. The developed antibodies were successfully applied to detect pyrrole-protein adducts in blood samples obtained from PA-treated rats and exhibited a potential for the clinical diagnosis of PA exposure.

References

Feb 24, 1968·Nature·A R Mattocks
Jul 1, 1996·Chemical Research in Toxicology·J Zheng, B D Hammock
Mar 1, 1996·Chemical Research in Toxicology·N C HalmesN R Pumford
Nov 17, 1998·Chemical Research in Toxicology·H E KleinerS S Lau
Aug 22, 2001·Journal of Agricultural and Food Chemistry·S T LeeR J Molyneux
Feb 3, 2007·Chemical Research in Toxicology·Wei YuanJiang Zheng
Apr 28, 2007·World Journal of Gastroenterology : WJG·Ning DaiJing Zhang
Mar 2, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·J A EdgarR J Molyneux
Mar 29, 2013·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Timothy ElvidgeBastiaan Hoogendoorn
Sep 21, 2013·Journal of Ethnopharmacology·Yan Hong LiGe Lin
Dec 12, 2013·Analytical and Bioanalytical Chemistry·Michalina OplatowskaKatrina Campbell
May 20, 2014·Chemical Research in Toxicology·Jianqing RuanGe Lin
Sep 16, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Dorina BodiAnja These
Dec 2, 2014·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Chia-Chi WangPeter P Fu
Sep 24, 2015·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Jianqing RuanGe Lin
Jan 14, 2016·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Xiaobo HePeter P Fu
Jan 27, 2016·Chemical Research in Toxicology·Weiwei LiJiang Zheng
Dec 19, 2016·Chemical Research in Toxicology·Hui WangJiang Zheng

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Citations

Apr 19, 2018·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Yan-Hong LiGe Lin
Jul 7, 2018·Journal of Food and Drug Analysis·Jiang MaGe Lin
Jun 6, 2020·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Zijing SongGe Lin

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Methods Mentioned

BETA
electrophoresis
protein assay
immunoprecipitation
ELISA

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