Immunoassay for monitoring environmental and human exposure to the polybrominated diphenyl ether BDE-47.

Environmental Science & Technology
Ki Chang AhnB D Hammock

Abstract

We developed a selective competitive enzyme-linked immunosorbent assay (ELISA) to monitor environmental and human exposure to polybrominated diphenyl ether BDE-47 that is used as a flame retardant 2,2',4,4'-Tetrabromodiphenyl ether (BDE-47), a dominant PBDE congener of toxicological concern, was the target analyte. To achieve effective hapten presentation on the carrier protein for antibody production, immunizing haptens with a rigid double-bonded hydrocarbon linker introduced at different positions on the target molecule were synthesized as well as coating haptens that mimic a characteristic fragment of the molecule. Rabbit antisera produced against each immunizing antigen were screened against competitive hapten coating antigens. Underoptimized competitive indirect ELISA conditions, the linear detection range in the assay buffer that includes 50% dimethyl sulfoxide was 0.35-8.50 microg/L with an IC50 value of 1.75 microg/L for BDE-47. Little or no crossreactivity (<6%) was observed to related PBDE congeners containing the BDE-47 moiety and other halogenated compounds. Using a magnetic particle-based competitive direct ELISA increased the sensitivity by 10-fold over the indirect ELISA. The ELISA provided quantitative results w...Continue Reading

References

Feb 1, 1993·Bulletin of Environmental Contamination and Toxicology·J P MapesS B Friedman
Sep 20, 2000·Journal of Agricultural and Food Chemistry·G ShanB D Hammock
Mar 17, 2001·Environmental Health Perspectives·P O DarnerudM Viluksela
Mar 21, 2002·Journal of Agricultural and Food Chemistry·Jae Koo LeeDae-Whang Kim
May 10, 2002·Chemosphere·Cynthia A de Wit
Apr 5, 2003·Analytical and Bioanalytical Chemistry·Jeanne V SamsonovaMilan Franek
Jul 5, 2003·Environmental Health Perspectives·Myrto PetreasM Judith Charles
May 12, 2005·Journal of Agricultural and Food Chemistry·Weilin L ShelverQing X Li
Oct 24, 2007·Environmental Science & Technology·Janice A DyeLinda S Birnbaum
May 6, 2008·Environment International·Joseph G AllenThomas F Webster
May 13, 2008·Environmental Health Perspectives·Kellyn S Betts
Jun 5, 2008·Environmental Science & Technology·Heather M StapletonDonald G Patterson
Jul 2, 2008·Environmental Science & Technology·Joseph G AllenThomas F Webster
Sep 3, 2008·International Archives of Occupational and Environmental Health·Kiyoung LeeMarc B Schenker
Dec 17, 2008·Environmental Health Perspectives·Mary E TurykHenry A Anderson
Mar 27, 2009·Neonatology·Nadia AbdelouahabLarissa Takser

❮ Previous
Next ❯

Citations

Aug 4, 2010·Analytical and Bioanalytical Chemistry·Lina KantianiDamià Barceló
Mar 5, 2016·Environmental Science & Technology·Ki Chang AhnShirley J Gee
Jun 5, 2010·Analytical Biochemistry·Ting XuJi Li
May 23, 2016·Analytical and Bioanalytical Chemistry·Candace S BeverShirley J Gee
Aug 28, 2016·Analytica Chimica Acta·Un-Jung Kim, Byoung Chan Kim
Jun 4, 2014·The Analyst·Francesc A Esteve-TurrillasAntonio Abad-Fuentes
Dec 12, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaohan Zhang, Huisheng Zhuang
Sep 26, 2013·Organic & Biomolecular Chemistry·Rosario López-MorenoAntonio Abad-Fuentes
Aug 5, 2010·Journal of Agricultural and Food Chemistry·Celia Suárez-PantaleónAntonio Abad-Fuentes
Oct 4, 2011·Analytical Chemistry·Anastasia MeimaridouMichel W F Nielen
Jul 30, 2014·ACS Sustainable Chemistry & Engineering·Rajeswaran RadhakrishnanBruce D Hammock

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.