Immunoblotting for the serodiagnosis of Helicobacter pylori infection in Brazilian patients with and without gastric carcinoma

Memórias do Instituto Oswaldo Cruz
Andreia Maria Camargos RochaD M Queiroz

Abstract

We evaluated the performance of a commercial immunoblotting in the serodiagnosis of Helicobacter pylori infection in Brazilian patients. The presence of anti-H. pylori antibodies was also investigated in a group of 20 duodenal ulcer patients after successful treatment. One hundred and ninety one patients were studied. Among the 164 infected patients, 46 had gastric carcinoma. The duodenal ulcer patients were treated with antimicrobial drugs and the eradication of the microorganism was confirmed in all of them one month after the end of the treatment by the 13C-urea breath test. Sera were assayed for H. pylori antibodies using the Helicoblot 2.0 (Genelabs Diagnostics, Singapore). The sensitivity, specificity, positive, and negative predictive values of the test were 93.9%, 92.6%, 98.7%, and 71.4%, respectively. The sensitivity of the test was similar in patients with (93.5%) and without (95.7%) gastric carcinoma. Twenty-four months after the end of the treatment, the band of 116 kDa was still detected in one of the patients. In conclusion, the Helicoblot 2.0 is an accurate test to diagnose H. pylori infection and although it can not be employed to monitor the bacterium eradication, it may be useful for diagnosing past infection,...Continue Reading

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Citations

Sep 24, 2005·Helicobacter·Karen A KrogfeltFrancis Mégraud
May 18, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Najmeh KaramiMarjan Mohammadi
Jun 12, 2013·Arquivos De Gastroenterologia·Luiz Gonzaga CoelhoJoaquim Prado P Moraes-Filho
Mar 16, 2018·The Cochrane Database of Systematic Reviews·Lawrence Mj BestKurinchi Selvan Gurusamy
Jul 26, 2018·Arquivos De Gastroenterologia·Luiz Gonzaga Vaz CoelhoJosé Vitor Zir

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