PMID: 8981655Nov 1, 1996Paper

Immunochemical and functional properties of biliary alpha-1-antitrypsin

Scandinavian Journal of Clinical and Laboratory Investigation
S JanciauskieneS Eriksson

Abstract

Alpha-1-antitrypsin (AAT), the archetype of the serpin (serine proteinase inhibitor) superfamily, is synthesized by hepatocytes and excreted to some extent into bile. The role of intact biliary AAT in gallstone pathogenesis is unsettled, but its 36-residue C-terminal peptide was found to promote cholesterol crystallization in a bile model. The present study showed biliary AAT to have specific properties that differ from serum AAT regarding immunoreactivity, heat stability and antiproteolytical activity. Electrophoretical and immunochemical methods were used to characterize biliary AAT. The level of its inhibitory activity was determined spectrophotometrically. In 18 samples from common bile duct and 12 samples from gallbladder bile, AAT was found to be heat-stable and functionally inactive. Added to the untreated, temperature-inactivated or protease inhibitors containing bile, native AAT became functionally inactive, heat-stable and lost its immunoreactivity. In contrast, heat-inactivated AAT, native albumin, transferrin, alpha-1-antichymotrypsin and IgG were unaffected on being added to bile. AAT in human bile manifests specific biochemical properties, such as functional inactivity and heat stability, that may be consistent wi...Continue Reading

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Citations

Nov 21, 1998·Journal of Hepatology·S JanciauskieneS Lindgren
Nov 15, 2002·Cancer Detection and Prevention·Inga ZelvyteSabina Janciauskiene
Dec 9, 2010·Alimentary Pharmacology & Therapeutics·F MihalacheV Zimmer
Mar 4, 2011·Respiratory Medicine·Sabina M JanciauskieneTobias Welte
May 25, 2002·The Journal of Biological Chemistry·Sabina JanciauskieneSten Eriksson
Mar 20, 2020·Biomedical Reports·Rafaat H Khalil, Nabil Al-Humadi
Apr 3, 2003·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·I ZelvyteS Janciauskiene

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