PMID: 7581276Jun 1, 1995Paper

Immunochemical characterisation and epitope mapping of a novel fimbrial protein (Pg-II fimbria) of Porphyromonas gingivalis

FEMS Immunology and Medical Microbiology
T OgawaM Hasegawa

Abstract

Mouse monoclonal antibody (mAb) Pgf-II specific for a 72-kDa major cell-surface protein (72K-CSP) derived from Porphyromonas gingivalis OMZ 409 was prepared. Immunoblotting analysis revealed that mAb Pgf-II reacted with 72K-CSP but not with 41-kDa fimbrial subunit protein (41K-fimbrilin) derived from P. gingivalis 381. Electron microscopic observation revealed that P. gingivalis OMZ 409 possessed peritrichous, thin fimbriae on their surface. Immunogold electron microscopy also demonstrated that mAb Pgf-II bound to the 72K-CSP examined with the gold particles arranged along the fibril array originating from the cell surface of the bacteria. These findings suggested that P. gingivalis 72K-CSP was identifiable as another fimbriae (termed Pg-II fimbriae) different from the fimbriae (termed Pg-I fimbriae) composed of a 41K-fimbrilin. Using multipin peptide synthesis technology, 102 sequential overlapping peptides covering the entire 514 amino-acid stretch of Pg-II fimbriae were synthesised. Seven immunodominant regions within Pg-II fimbrial protein molecule, which definitely reacted with the serum of patients with periodontal diseases, were detected.

References

Jun 1, 1979·Biophysical Journal·P Y Chou, G D Fasman
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Feb 21, 1991·Nature·D C Feller, V F de la Cruz
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Citations

Mar 27, 1999·Oral Microbiology and Immunology·S HamadaI Morisaki
Jun 24, 2005·Infection and Immunity·Yoonsuk ParkRichard J Lamont
Nov 1, 1996·Annals of Periodontology·S Offenbacher
May 10, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Wu, P M Fives-Taylor
Aug 3, 2007·Expert Review of Vaccines·Dileep C G SharmaBangalore Varadhan Karthikeyan
Aug 21, 2020·Microbiology and Immunology·Mikio ShojiKoji Nakayama
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·R J Lamont, H F Jenkinson

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