PMID: 1420280Oct 27, 1992

Immunochemical characterization and toxicological significance of P-450HFLb purified from human fetal livers

Biochimica Et Biophysica Acta
M KitadaY Kanakubo

Abstract

Immunochemical properties of P-450HFLb purified from human fetal livers were investigated. P-450HFLb cross-reacted with antibodies to rat P-4501A1 but not with antibodies to CYP2A6, CYP2C9, CYP3A7 (P-450HFLa) and rat CYP2B1. In addition, P-450HFLb also cross-reacted with both monospecific antibodies to rat CYP1A1 and CYP1A2. However, P-450HFLb was shown to be an immunochemically distinct form of cytochrome P-450 from P-450PA (human CYP1A2). Immunoblot analysis of human fetal livers with the antibodies to P-450HFLb showed that P-450HFLb was expressed in all fetal livers studied although there appeared to be individual differences in the amounts of P-450HFLb expressed in fetal livers. The formation of mutagens from IQ (but not from AFB1) in fetal liver homogenates was inhibited by the antibodies to P-450HFLb in a dose dependent manner. These results suggest that P-450HFLb may be a form of human cytochrome P-450 classified into CYP1 gene family, and that the cytochrome P-450 is, in part, responsible for the mutagenic activation of IQ in human fetal livers as well as CYP3A7 (P-450HFLa).

References

Jan 1, 1990·Pharmacology & Therapeutics·F J Gonzalez
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L C QuattrochiR H Tukey
Oct 22, 1970·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·S J YaffeS Orrenius
Jul 1, 1980·Clinical Pharmacokinetics·M R JuchauC J Omiecinski

Citations

Nov 25, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·E TanakaT Horie
Jul 11, 2007·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A MadanH P Bozigian
Mar 11, 2000·Drug Metabolism Reviews·R Kato, Y Yamazoe
Jan 1, 1994·Drug Metabolism Reviews·M Kitada, T Kamataki
Dec 25, 2004·Drug Metabolism and Pharmacokinetics·Tamihide MatsunagaMitsukazu Kitada
Dec 31, 1997·Microscopy Research and Technique·K J Rich, A R Boobis

Related Concepts

2-Amino-3-methylimidazo[4,5-f]quinoline
Metazoa
Metabolic Biotransformation
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Cytochrome P-450 Oxygenase
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Quinolines
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