PMID: 9529066Apr 7, 1998Paper

Immunochemical characterization of the MPB70/80 and MPB83 proteins of Mycobacterium bovis

Infection and Immunity
H G WikerM Harboe

Abstract

MPB70 and MPB80 (MPB70/80) and MPB83 are closely related antigens which are highly expressed in Mycobacterium bovis. MPB70/80 are soluble secreted antigens, while MPB83 is an exported lipoprotein associated with the bacterial surface. In the present study, these antigens had different mobilities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing and nonreducing conditions. These differences may be explained by the fact that MPB70 and MPB83 both have two internal cysteine residues which would create ring structures by disulfide bonding. We analyzed the structures of MPB70/80 and MPB83 by using monoclonal antibodies (MAbs) raised against bovine purified protein derivative or whole M. bovis cells. MAb 1-5C reacted specifically with MPB70 and MPB80, and MAb MBS43 reacted specifically with MPB83, while the other antibodies, including several previously described MAbs, bound all three antigens. MAbs and polyclonal antibodies reacted strongly with reduced protein and less well with nonreduced protein, indicating involvement of linear epitopes. Epitopes of MAbs Bov-1, 2-6B, 1-5C, and 1-1D were mapped by using synthetic peptides of MPB70. Sequence comparison showed the peptide with the 1-5C-reactive epitope to h...Continue Reading

References

Mar 1, 1992·Veterinary Microbiology·T FifisP R Wood
Dec 1, 1990·Immunology and Cell Biology·H Billman-JacobeP R Wood
Apr 1, 1986·Infection and Immunity·M HarboeN Cruz
Mar 1, 1981·Infection and Immunity·S NagaiT Nagasuga
Jul 1, 1995·Scandinavian Journal of Immunology·M HarboeS Haga
May 1, 1996·Scandinavian Journal of Immunology·T MatsuoT Yamada
Jan 10, 1998·Infection and Immunity·M HarboeS Nagai

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Citations

May 15, 2009·Scandinavian Journal of Immunology·H G Wiker
Jul 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shajo Kunnath-VelayudhanMaria L Gennaro
Aug 12, 2014·BioMed Research International·María Laura MonMaría Isabel Romano
Nov 29, 2002·Preparative Biochemistry & Biotechnology·Elvira VarelaLuis F Montaño
Aug 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rachel J DuttonJon Beckwith
May 9, 2012·Veterinary Medicine International·Scott D FitzgeraldJohn B Kaneene
Jul 31, 2009·Clinical and Vaccine Immunology : CVI·V MeikleA Cataldi
Apr 20, 2001·Scandinavian Journal of Immunology·K P LyashchenkoJ M Pollock
Nov 13, 2004·FEMS Microbiology Reviews·Iain C Sutcliffe, Dean J Harrington
Feb 18, 2003·Scandinavian Journal of Immunology·S TollefsenH G Wiker
Apr 18, 2000·Infection and Immunity·S G RhodesH M Vordermeier
Oct 15, 2003·FEMS Immunology and Medical Microbiology·Aharona Glatman-Freedman
May 8, 2003·Journal of Clinical Microbiology·J M PollockP Andersen

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