PMID: 810521Oct 1, 1975

Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis

The Journal of Infectious Diseases
D L Kasper, M W Seiler

Abstract

The outer membrane of gram-negative bacteria is immunologically the most important structure of the cell because of its accessibility to host defense mechanisms. By electron micrographic studies, it has been shown that Bacteroides fragilis has a cell envelope typical of gram-negative bacteria. The outer memberane of this envelope was found to consist of protein, two polysaccharides bound to lipid in various degrees, and loosely bound lipid. The protein component of the outer membrane had a distinct pattern when studied by polyacrylamide gel electrophoresis. Similar peptide band pattern were seen in all strains of Bacteroides fragilis subspecies fragilis studied, but this pattern was distinct from the pattern found in the outer membranes of other subspecies. The carbohydrates and the protein components were both associated with subspecies-specific immunoprecipitins. We propose that this antigenic complex represents the outer membrane of B. fragilis in its native state and that the immunologically important antigens of this organism are associated with this complex.

Citations

Jul 1, 1994·World Journal of Microbiology & Biotechnology·T KhannaS Majumdar
Nov 1, 1994·World Journal of Microbiology & Biotechnology·T KhannaS Majumdar
Jan 1, 1980·Transactions of the Royal Society of Tropical Medicine and Hygiene·J E Sippel, A Voller
Jun 1, 1978·The Journal of Hygiene·K M Elhag, S Tabaqchali
Feb 1, 1989·Antimicrobial Agents and Chemotherapy·Y MutoK Ueno
May 1, 1976·Antimicrobial Agents and Chemotherapy·B OlssonT Wadström
Nov 1, 1978·Journal of Clinical Pathology·C A Ross, R F Gilmore
Jan 1, 1994·Antonie van Leeuwenhoek·I BeckmannH C Wallenburg
Oct 1, 1984·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·A C Rodloff, H Hahn
Nov 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·S EhlersH Hahn
Dec 1, 1984·The Journal of Applied Bacteriology·F Meisel-MikołajczykJ Paelinck
May 20, 1998·The Journal of Surgical Research·T T SatoT H Pohlman
Jan 1, 1987·Critical Reviews in Microbiology·I Brook
Feb 1, 1977·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·T Hofstad
Feb 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·A SchwanU Forsum

Related Concepts

Antigens, Bacterial
Bacterial Proteins
Bacteroides fragilis
Plasma Membrane
SDS-PAGE
Neisseria meningitidis

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