Immunochemical detection of a common antigen among Streptococcus uberis isolates.

Journal of Clinical Microbiology
K F Jones, N L Norcross

Abstract

Fifty-three isolates of Streptococcus uberis from various sources were examined for the presence of a common antigen. Initially, a serum was produced in rabbits which, by using rocket line immunoelectrophoresis, proved to react with identity to all of the S. uberis crude extracts as well as group B and E streptococcal extracts. The antigen(s) responsible for this cross-reactivity was partially purified by Sephacryl S-200 gel chromatography and analyzed by fused rocket immunoelectrophoresis. Further analysis by immunodiffusion showed that probably two antigens in the gel chromatography-consolidated fractions were common to the S. uberis and group B and E isolates, but that one of the antigens present was unique to S. uberis. Trypsin destroyed the immunoreactivity of this antigen. Isolation of this common antigen could possibly alleviate some of the tedium associated with the identification of this organism.

References

Jan 1, 1976·Infection and Immunity·C J Baker, D L Kasper
Jan 1, 1977·Journal of Immunological Methods·A J Crowle, L J Cline
Jun 1, 1973·Bacteriological Reviews·K W Knox, A J Wicken
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·P J Svendsen
Mar 31, 1933·The Journal of Experimental Medicine·R C Lancefield

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Citations

Aug 1, 1986·The Journal of Dairy Research·J R Tagg, L G Vugler
Dec 1, 1991·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·C Lämmler
Oct 1, 1991·Epidemiology and Infection·M H GroschupJ F Timoney

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