PMID: 7524614May 1, 1994Paper

Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis

British Journal of Haematology
S J ThorpeE R Huehns

Abstract

A fixation and permeabilization procedure using formaldehyde and acetone has been developed which allows immunostaining of intracellular haemoglobin for fluorescence activated cell sorter (FACS) analysis of erythrocytes. The treatment preserves antigenicity and light-scattering properties. Validation of the method was given by the correlation of F cell number in adults determined by FACS analysis with that assessed by microscopic examination of cell smears, and by the direct relationship between beta chain synthesis and intensity of beta chain/Hb A immunofluorescence within fetal erythrocyte samples known to vary in their beta chain/Hb A content. The procedure is rapid, non-subjective and sensitive, and makes analysis of haemoglobin content, type and distribution amongst red cell populations possible.

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·H ChangD G Nathan
Oct 1, 1987·Journal of Molecular and Cellular Cardiology·S Sugiyama, T Ozawa
Oct 1, 1980·British Journal of Haematology·M MatsakisN Sakarellou
Dec 12, 1959·Nature·K BETKEI SCHLICHT
Jan 1, 1992·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·H B TanowitzM Wittner

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Citations

Mar 20, 2003·Experimental Hematology·Evangelia SkarpidiGeorge Stamatoyannopoulos
Dec 16, 1998·Vox Sanguinis·M NelsonJ Gibson
May 1, 1999·Human Gene Therapy·D W EmeryG Stamatoyannopoulos
Oct 8, 2009·Human Molecular Genetics·Swee Lay TheinChad Garner
Mar 1, 1995·British Journal of Haematology·M GanczakowskiD J Weatherall
Jul 21, 1998·Annals of the New York Academy of Sciences·N F OlivieriD J Weatherall
May 19, 2010·Molecular and Cellular Biology·Karina LaflammeDavid M Bodine
Nov 26, 2010·The Journal of Clinical Investigation·Patrick G GallagherDavid M Bodine
Jul 28, 2005·Journal of Inflammation·Jennifer M RyanBernard P Mahon
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Swee Lay TheinMark Lathrop
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·D E SabatinoD M Bodine
Jun 7, 2011·Blood Cells, Molecules & Diseases·Michael S BoosalisSusan P Perrine
Nov 25, 2003·British Journal of Haematology·Argirios TsantesGeorgios Paterakis
Jun 29, 2006·British Journal of Haematology·Tamon NishinoDavid W Emery
Nov 17, 2006·British Journal of Haematology·Jackie Sloane-StanleyWilliam G Wood
Nov 9, 2007·International Journal of Laboratory Hematology·K Y ItaliaD Mohanty
Apr 7, 2009·British Journal of Haematology·Swee Lay Thein, Stephan Menzel
Jul 30, 2014·Annals of Human Genetics·Stephan MenzelSwee Lay Thein
Dec 1, 1994·Blood Reviews·J RochetteS L Thein
Jul 4, 2006·Blood Cells, Molecules & Diseases·Tamon NishinoDavid W Emery
Sep 1, 2015·Nature Methods·Jeff VierstraJohn A Stamatoyannopoulos
Mar 31, 2012·Blood·Diamantis G KonstantinidisTheodosia A Kalfa
Apr 2, 2008·IUBMB Life·Laura Manca, Bruno Masala
Jul 1, 1997·Irish Journal of Medical Science·T C ThamJ S Collins
Sep 27, 2019·Journal of Immunoassay & Immunochemistry·Lamplaimas TangpanThanusak Tatu

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