Immunochemical evidence for the enzymatic difference of delta 6-desaturase from delta 9- and delta 5-desaturase in rat liver microsomes

Biochemical and Biophysical Research Communications
Y FujiwaraY Imai

Abstract

The enzymatic properties of the three types of microsomal acyl-CoA desaturases, delta 6-, delta 9- and delta 5-desaturases, were immunologically compared using a monospecific antibody raised against the purified linoleoyl-CoA desaturase (delta 6-desaturase). By the double immunodiffusion technique, the anti-delta 6-desaturase antibody showed a single precipitin line to the purified delta 6-desaturase and microsomes treated with Triton X-100, but no line was observed with the partially purified delta 9-desaturase. The antibody even inhibited definitely delta 6-desaturase activity in microsomes, but neither stearoyl-CoA (delta 9-) nor eicosatrienoic acid (delta 5-) desaturations were inhibited. By these immunological investigations it was confirmed that terminal delta 6-desaturase is different enzyme from desaturases delta 9- and delta 5.

References

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Apr 1, 1972·Archives of Biochemistry and Biophysics·N Oshino, R Sato
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Citations

Jan 1, 1988·Progress in Lipid Research·V A Ziboh, R S Chapkin
Sep 1, 1984·Archives of Biochemistry and Biophysics·Y FujiwaraY Imai

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