PMID: 3768727Oct 1, 1986Paper

Immunochemical homology of 3 developmentally regulated brain proteins and their developmental change in neuronal distribution

Brain Research
Tomoaki Shirao, K Obata

Abstract

Proteins S5, S6, and S54 (mol. wts. 95,000, 100,000, and 110,000 Da) appear characteristically at certain developmental stages in the chick brain (Shirao, T. and Obata, K., J. Neurochem., 44 (1985) 1210-1216). In the present study polyclonal and monoclonal antibodies were developed against electrophoretically purified S5 and S6 proteins. Each polyclonal and monoclonal antibody specifically recognized all 3 proteins, S5, S6, and S54, by immunoblot analysis. The tissue specificities of these proteins were examined by immunoblot analysis with these antibodies. Proteins S5 and S6 were found in the neural tissue and in some non-neural tissues of chick embryo. In the adult chicken, however, they were detected neither in neural nor in non-neural tissues with the exception of the spinal ganglion. Protein S54, on the other hand, was found both in late embryonic and adult neural tissues. It was detected neither in embryonic nor in adult non-neural tissues. Immunohistochemical analysis of adult nervous system showed that S54 protein was present only in neurons. Therefore it is concluded that S54 protein is a neuron-specific protein. Developmental changes of localization of these proteins were then examined by immunohistochemistry. In the ...Continue Reading

References

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Citations

Feb 26, 1996·Brain Research. Developmental Brain Research·K IkedaT Shirao
Jan 1, 1990·Neuroscience Research. Supplement : the Official Journal of the Japan Neuroscience Society·T ShiraoK Obata
Nov 7, 1998·Brain Research. Developmental Brain Research·K HayashiT Shirao
Oct 2, 2001·Neuroscience Letters·S KobayashiT Sasaki
Jun 26, 2007·Neurochemistry International·Yuko SekinoTomoaki Shirao
Feb 19, 2009·Journal of Neurochemistry·Toshiyuki MizuiTomoaki Shirao
Dec 18, 2012·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Haruo NishijimaMasahiko Tomiyama
Oct 15, 2013·Journal of Neurochemistry·Hiroyuki YamazakiTomoaki Shirao
Sep 9, 2015·Journal of Neuroscience Research·Yuki OharaTomoaki Shirao
Feb 7, 2012·Molecular and Cellular Neurosciences·Xin-peng DunJohn K Chilton
Mar 30, 2016·Radiation Research·Anggraeini PuspitasariTomoaki Shirao
Apr 24, 2015·PloS One·Takahiko ChimuraNobuaki Yoshida
Oct 12, 2001·The European Journal of Neuroscience·H YamazakiT Shirao
Jan 1, 1993·Cell Motility and the Cytoskeleton·E L de HostosG Gerisch
Feb 16, 2017·Journal of Neurochemistry·Tomoaki ShiraoYuko Sekino
Jun 15, 2007·The Journal of Comparative Neurology·Chiho KobayashiTomoaki Shirao
Mar 15, 2008·Journal of Neuroscience Research·Carl JulienFrédéric Calon
May 23, 2013·The Journal of Biological Chemistry·Mouna A MikatiEmil Reisler
Aug 25, 2016·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Seung Hoon LeeYoung-Joo Won
Jul 15, 2020·Epilepsia·Thaís Caroline Dallabona DombroskiAntonio Roberto Martins
Aug 24, 2017·The European Journal of Neuroscience·Yuki KajitaTomoaki Shirao

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