PMID: 2428914Oct 1, 1986Paper

Immunochemical localization and amino acid sequences of crossreactive epitopes within the group A streptococcal M6 protein

The Journal of Experimental Medicine
K F JonesVincent A Fischetti

Abstract

mAbs 10A11, 10B6, and 10F5, raised against the native group A streptococcal M6 protein, were examined for their crossreactivity with non-laboratory passaged clinical isolates, representing 58 M serotypes, by bacterial dot blot immunoassay. mAb 10A11 crossreacted with 9, mAb 10B6 with 30, and mAb 10F5 with 30 different non-M6 serotypes. To identify the epitopes for these antibodies, the native M6 protein was cleaved with pepsin or staphylococcal V8 protease. Resultant peptides were purified by HPLC, examined for binding to crossreactive mAbs in ELISA, and reactive peptides were subjected to amino acid sequence analysis. Peptides were aligned with the amino acid sequence of the entire M6 protein predicted by the DNA sequence of the M6 gene. Competitive inhibition studies using peptides synthesized on the basis of peptide and DNA sequences, in concert with selective blocking of amino acid residues, allowed for the further identification and placement of these crossreactive epitopes within the M6 molecule. The 10A11 epitope was located within the six amino acid residues at position 134-139, which repeat at positions 159-164 and 184-189 within the variable amino terminal half of the native molecule. The conserved 10B6 and 10F5 epito...Continue Reading

References

Mar 1, 1985·The Journal of Experimental Medicine·K F JonesV A Fischetti
Nov 1, 1969·The Journal of Experimental Medicine·J SwansonE C Gotschlich
Aug 19, 1983·Science·J R Scott, V A Fischetti
Dec 16, 1983·Biochemical and Biophysical Research Communications·M JolivetL Chedid
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E H BeacheyL Chedid
Jan 29, 1980·Biochemical and Biophysical Research Communications·J M SeyerE H Beachey
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N PhillipsV A Fischetti

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Citations

Sep 1, 1988·European Journal of Epidemiology·B N Manjula
Sep 1, 1992·Immunology Today·J H Robinson, M A Kehoe
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K F JonesV A Fischetti
Jan 1, 1989·The Journal of Experimental Medicine·D BessenV A Fischetti
Aug 31, 2006·The Pediatric Infectious Disease Journal·Thomas B MartinsHarry R Hill
Nov 1, 1991·The British Journal of Dermatology·J McFaddenL Fry
Dec 1, 1991·Molecular Microbiology·K F JonesV A Fischetti
Jul 25, 2012·Infection and Immunity·Hwan Keun KimOlaf Schneewind
Mar 27, 2002·Journal of Bacteriology·Timothy C Barnett, June R Scott
May 1, 1993·Molecular Microbiology·S K HollingsheadD E Bessen
Jun 7, 2005·Advanced Drug Delivery Reviews·Rebecca L Wilson, Dennis E Hruby
Jul 19, 2013·Human Vaccines & Immunotherapeutics·Michael F GoodManisha Pandey
Aug 22, 2001·Preparative Biochemistry & Biotechnology·D M MyscofskiD E Hruby
Feb 28, 1998·The Journal of Biological Chemistry·Y Chien, A L Cheung
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nadia M J EllisMadeleine W Cunningham
Jan 12, 1999·Protein Expression and Purification·D M Myscofski, D E Hruby
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind
Jul 1, 1989·Clinical Microbiology Reviews·V A Fischetti

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