Immunochemical localization of the epitope for a monoclonal antibody that neutralizes human platelet-derived growth factor mitogenic activity.

Molecular and Cellular Biology
W J LaRochelleS A Aaronson

Abstract

A monoclonal antibody (mAb), sis 1, generated against human c-sis-encoded platelet-derived growth factor (PDGF) BB, was shown by enzyme-linked immunosorbent assay and Western blot (immunoblot) analysis to recognize human PDGF BB and human platelet PDGF AB but not the human PDGF AA. This monoclonal antibody potently inhibited PDGF receptor-binding and mitogenic activities of both human PDGF BB and PDGF AB but had no effect on PDGF AA. Finally, we demonstrated that an immunoaffinity-purified anti-c-sis peptide antibody (anti-V4) which also blocked binding of PDGF BB to its cognate receptor and competed with mAb sis 1 for binding to PDGF BB. All of these results suggest that mAb sis 1 recognizes an epitope of the c-sis gene product, PDGF BB, that spatially overlaps the V4 surface domain of PDGF BB, immunochemically localizing a region of PDGF BB critical for PDGF receptor binding and activation.

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Citations

Oct 1, 1992·Pathology·L M Khachigian, C N Chesterman
Jul 5, 2005·Pharmacology & Therapeutics·Maria Burian, Gerd Geisslinger
Jan 12, 2013·Brain, Behavior, and Immunity·Johan RuudAnders Blomqvist
Jun 20, 2012·The Journal of Pharmacology and Experimental Therapeutics·Zhi-Ming HaoHong-Hong Li
Nov 6, 2001·The Journal of Biological Chemistry·Katia RussoAntonio Facchiano
Sep 30, 2003·Journal of the American Society of Nephrology : JASN·Sekiko TanedaCharles E Alpers
Jun 25, 1993·Biochimica Et Biophysica Acta·B HerrenM Pech

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