PMID: 6404974May 1, 1983Paper

Immunochemical partial identity between two independently identified and isolated major allergens from Alternaria alternata (Alt-I and Ag 1)

The Journal of Allergy and Clinical Immunology
L NyholmJ W Yunginger

Abstract

Partially purified preparations of Alt-I, the main allergenic fraction of Alternaria alternata isolated by Yunginger, and of Ag 1, shown in crossed radioimmunoelectrophoresis (CRIE) to be the dominating major allergen of A. alternata (Løwenstein, Nyholm), were compared by tandem crossed immunoelectrophoresis (CIE), RAST inhibition, and the CRIE-related technique, single radial radioimmunodiffusion (SRRID). The two allergen preparations showed reaction of identity in tandem-CIE and indistinguishable specific IgE binding in CRIE and SRRID, regardless of antibodies and serum pools used. In RAST inhibition, the relative potencies of the allergen preparations and of the crude extracts correlated well with their Alt-I/Ag 1 content as estimated by rocket immunoelectrophoresis. Moreover, all inhibition curves were parallel, confirming identical IgE binding by Alt-I and Ag 1 with the serum pools used. A second preparation of Alt-I, isolated from another strain of Alternaria, showed reaction of partial identity with Ag 1 in tandem-CIE, indicating that different variants of Alt-I (Ag 1) may exist in different strains of A. alternata.

References

Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Dec 1, 1982·The Journal of Allergy and Clinical Immunology·M K AgarwalJ W Yunginger
Aug 1, 1980·The Journal of Allergy and Clinical Immunology·J W YungingerM Geller

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Citations

Feb 23, 1994·Molecular and Cellular Biochemistry·J VermaS V Gangal
Apr 1, 1991·Molecular Immunology·M J Deards, A E Montague
Mar 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J Kleine-TebbeG Kunkel
Jun 11, 2005·Allergologia et immunopathologia·H BouzianeB David
Nov 1, 1986·The Journal of Allergy and Clinical Immunology·H LowensteinB Schwartz
Sep 14, 1999·The Journal of Allergy and Clinical Immunology·R K BushD Geisler
Apr 1, 1988·The Journal of Allergy and Clinical Immunology·R M HelmJ W Yunginger
Dec 1, 1991·The Journal of Allergy and Clinical Immunology·S ParisJ P Latgé
May 18, 2018·Bioanalysis·Maria A SimonovaElena V Svirshchevskaya
Oct 1, 1987·Postgraduate Medicine·R P Stankus, S B Lehrer
Mar 9, 2007·Annals of Saudi Medicine·S M HasnainS Al-Sedairy
Apr 1, 1995·Clinical Microbiology Reviews·W E HornerS B Lehrer

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