Immunochemical quantification of crossline as a fluorescent advanced glycation endproduct in erythrocyte membrane proteins from diabetic patients with or without retinopathy

Diabetic Medicine : a Journal of the British Diabetic Association
M YamaguchiM Kondo

Abstract

Crossline is a novel advanced glycation endproduct (AGE) which has both a crosslink and fluorescence similar to AGE-protein in vivo. To assess the association of AGEs to the development of diabetic retinopathy we developed a sensitive and specific enzyme-linked immunosorbent assay (ELISA) for crossline in blood samples and investigated the association of the development of retinopathy and erythrocyte membrane protein (EMP)-crossline concentrations in patients with Type 2 diabetes mellitus (Type 2 DM). Crossline formation in EMP exceeded that in haemoglobin and was detectable in normal EMP samples without pretreatment by this ELISA system. Mean (+/-SE) EMP crossline levels were elevated 1.6-fold in diabetic patients without retinopathy (7.6 +/- 0.5 pmol mg(-1), p < 0.005), 2.2-fold in diabetic patients with non-proliferative retinopathy (10.5 +/- 0.6 pmol mg(-1), p < 0.001) and 2.6-fold in diabetic patients with proliferative retinopathy (12.0 +/- 0.6 pmol mg(-1), p < 0.001) compared with healthy control subjects (4.7 +/- 0.5 pmol mg(-1)). Type 2 DM patients with retinopathy had significantly higher EMP-crossline levels than those without retinopathy (p < 0.005). Our data suggest that elevated EMP-crossline concentrations are as...Continue Reading

Citations

Jul 24, 2010·Journal of Ophthalmology·Masayoshi TakeuchiSho-Ichi Yamagishi
Jul 23, 2005·Annals of the New York Academy of Sciences·Vincent M MonnierSaul Genuth
Jul 23, 2005·Annals of the New York Academy of Sciences·Alan W Stitt
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Jan 16, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ioannis K PetropoulosIssidora S Papassideri
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Apr 17, 2008·Journal of Diabetes and Its Complications·Balaji AnithaMohan Rema
Sep 28, 2010·Progress in Retinal and Eye Research·Chung-Jung Chiu, Allen Taylor

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