Immunochemical staining of the rat adenohypophysis in organ culture

The Anatomical Record
A S PakurarJ L Conklin

Abstract

Rat anterior pituitaries were cytologically studied following cultivation in organ culture, with and without the addition of hypothalamic and cortical extracts. Although five distinct cell types could be identified with classical stains in the uncultivated glands, the peroxidase-labeled antibody technique (using antibodies against STH, LTH, FSH, LH and TSH) showed that not all of the immune-specific cell types were being identified with the classical stains. This discrepancy was magnified following culture as chromophilic cells seen with classic stains decreased in number with an increase in culture time. The peroxidase technique, however, revealed that all cells remained constant in type and number regardless of time in culture. While the addition of either hypothalamic or cortical extract to the culture medium produced cytological alterations demonstrated by the classical dyes, the antibody technique showed no such alterations. Such a comparison of staining techniques emphasizes the hazards of relying solely on histological procedures to reveal the hormonal activity of the pituitary gland.

Citations

Jan 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane
Jun 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A E SwopeJ L Conklin
Jan 1, 1973·Acta Endocrinologica. Supplementum· zur Mühlen A vonJ Köbberling
Jan 1, 1972·Zeitschrift für Zellforschung und mikroskopische Anatomie·K Yamashita
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Jul 1, 1973·Endocrinology·S Kanematsu, C H Sawyer
Apr 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·J C MittlerA V Schally
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May 1, 1967·The Journal of Cell Biology·P K Nakane, G B Pierce
Jun 1, 1954·Endocrinology·J W EVERETT
Jan 1, 1957·Zeitschrift für Zellforschung und mikroskopische Anatomie·E G RENNELS
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Dec 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, G B Pierce

Related Concepts

Ovoperoxidase
Organ Culture Techniques
Organum Vasculosum Laminae Terminalis
Peroxidases
Luteozyman
Tissue Extracts
Structure of Cortex of Kidney
Thyrotropin
Pituitary Gland, Anterior
Neoplasm of Uncertain or Unknown Behavior of Pituitary Gland

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