Dec 10, 1977

Immunochemical studies of fragments of bovine serum albumin

The Journal of Biological Chemistry
T PetersR G Reed

Abstract

Antigenic properties of 14 fragments of bovine albumin were measured using antisera to albumin and to two of its fragments. All seven fragments larger than 21,000 daltons formed immune precipitates. Although immune precipitates were not formed with smaller fragments, inhibition tests indicated the presence of antigenic sites on several of these fragments as well. The results predict the occurrence of six or more antigenic determinants and allow assignment of their positions in the parent molecule. These sites are distributed along the entire protein chain, with the sites of greatest antibody affinity situated in the COOH-terminal region. Evidence is presented that some sites are homologous, reacting with the same populations of antibodies, and that other sites are unique, binding to an exclusive population of antibodies.

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Mentioned in this Paper

Precipitin Tests
Antigenic Specificity
ALB
Immune Sera
Bos taurus
Peptide Fragments
Antigen-Antibody Reactions
Antibody Affinity
Metabolic Inhibition
Immune Precipitates

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