Immunochemical studies of plasma kallikrein

The Journal of Clinical Investigation
A BagdasarianR W Colman

Abstract

A monospecific antibody against human plasma kallikrein has been prepared in rabbits with kallikrein further purified to remove gamma globulins. The antisera produced contained antikallikrein and also anti-IgG, in spite of only 8% contamination of kallikrein preparation with IgG. The latter antibody was removed by adsorption of antisera with either Fletcher factor-deficient plasma or with purified IgG. Both kallikrein and prekallikrein (in plasma) cross-react with the antibody with no apparent difference between the precipitation arcs developed during immunoelectrophoresis and no significant difference in reactivity when quantified by radial immunodiffusion. Kallikrein antibody partially inhibits the esterolytic and fully inhibits the proteolytic activity of kallikrein. In addition, the antibody inhibits the activation of prekallikrein, as measured by esterase or kinin release. The magnitude of the inhibition is related to the molecular weight of the activator used. Thus, for the four activators tested, the greatest inhibition is observed with kaolin and factor XII(A), while large activator and the low molecular weight prekallikrein activators are less inhibited. With the kallikrein antibody, the incubation of kallikrein with e...Continue Reading

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Citations

Aug 1, 1976·Archives of Biochemistry and Biophysics·B LahiriR W Colman
May 1, 1981·Journal of the Neurological Sciences·L H DickermanR G Macbride
Jan 1, 1986·Critical Reviews in Oncology/hematology·R W Colman, A H Schmaier
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Related Concepts

Anthropoidea
Antibody Specificity
Molecular Sieve Chromatography
Canis familiaris
Electrophoresis, Disc
Enzyme Activation
Enzyme Precursors
Esterases
Factor XII
Gamma globulin

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