Immunochemical studies on a polysaccharide from Shigella dysenteriae type 2

Molecular Immunology
S BasuS G Dastidar

Abstract

The polysaccharide isolated from Shigella dysenteriae type 2, strain NCTC 566, on Smith degradation and graded hydrolysis yielded three oligosaccharides which were characterised using methylation studies. Using homologous rabbit antiserum and the monosaccharides that constituted the polysaccharide and the oligosaccharides isolated from it and the cross-reactions in some type-specific pneumococcal antisera, immunochemical specificities of different sugar groupings in the polysaccharide molecule were determined. These results indicated that N-acetylglucosamine was the immunodominant sugar in the polysaccharide and the oligosaccharide isolated from the Smith-degraded product and having the structure (formula; see text) gave maximum inhibition of the specific precipitation.

References

Apr 1, 1994·Epidemiology and Infection·G SamonisY Tselentis

Related Concepts

Precipitin Tests
Protein Methylation
Dysentery, Bacillary
Immune Sera
Cross Reactions
Polysaccharides, Bacterial
Methylation
Metabolic Inhibition
Polyvalent pneumococcal vaccine
Oligosaccharides

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