PMID: 6165051Dec 1, 1980Paper

Immunochemical studies on big gastrin using NH2-terminal specific antisera

Regulatory Peptides
G Dockray

Abstract

Methods are described for obtaining antisera specific for the NH2-terminal regions of human and porcine big gastrin (G34) that can be used in radioimmunoassays. Three antisera have been characterized in detail: one (L66) raised to human 1--15 (Tyr7-Pro8-Ser9) G34 has an antigenic determinant in the 1--6 region of human G34; a second (L107) raised to 1--19 hG34 has an antigenic determinant in the 1--12 region. Both these antisera react weakly with porcine G34. A third antiserum (L33) raised to porcine G34 has an antigenic determinant in the 1--12 region of this peptide, and reacts weakly with human G34. In human antral extracts fractionated on Sephadex G50, L66 and L107 revealed a minor peak of immunoreactivity corresponding to G34, and a major peak corresponding to the NH2-terminal tryptic peptide of G34. Concentrations of the latter peptide were closely similar to those of G17 (i. e.) the COOH-terminal tryptic peptide of G34), consistent with the idea that G34 is cleaved within G-cells by a trypsin-like enzyme to yield G17. Antiserum L33 revealed small amounts of immunoreactivity in antral extracts of dog and cat, but did not reveal significant immunoreactivity in rat antral extracts. In contrast, L66 reacted with rat antral e...Continue Reading

Citations

Nov 15, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·W W van Solinge, J F Rehfeld
Jan 1, 1986·Neurochemistry International·R G WilliamsG J Dockray
Oct 1, 1983·Journal of Biological Standardization·R K GuptaS N Saxena
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T W Wong, A R Goldberg
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I LaxJ Schlessinger
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J C LoftusM H Ginsberg
Jan 1, 1994·Journal of Gastroenterology and Hepatology·T S Gaginella
May 1, 1984·The Journal of Clinical Investigation·V E EysseleinJ H Walsh
Feb 1, 1995·Cell Adhesion and Communication·J H PetersR O Hynes
Jan 1, 1985·Annals of the New York Academy of Sciences·G J DockrayR G Williams
Dec 1, 1990·Scandinavian Journal of Gastroenterology·L Bardram
Feb 15, 1985·International Journal of Cancer. Journal International Du Cancer·R M KrisA Raz

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