PMID: 6403044Jan 25, 1983Paper

Immunochemical studies on cytochrome P-450 in adrenal microsomes

Biochimica Et Biophysica Acta
S KominamiS Takemori

Abstract

An antibody was prepared against electrophoretically homogeneous cytochrome P-450C21 purified from bovine adrenal microsomes. This antibody was used to compare various cytochromes P-450 in bovine and guinea pig adrenal microsomes. In an Ouchterlony double diffusion test, a spur formation was observed between the precipitin lines of the purified bovine cytochrome P-450C21 and guinea pig adrenal microsomes against anti-cytochrome P-450C21 IgG. Anti-cytochrome P-450C21 IgG inhibited 21-hydroxylation both of bovine and guinea pig adrenal microsomes but the inhibition was much more effective in the bovine microsomes than in the guinea pig microsomes. These results suggest that the 21-hydroxylase in the guinea pig microsomes has some molecular similarities to the bovine cytochrome P-450C21 and a part of the antibodies cross-reacts with the 21-hydroxylase in the guinea pig microsomes. Anti-cytochrome P-450C21 IgG did not inhibit the activities of 17 alpha-hydroxylase and C17,20-lyase in the bovine and guinea pig microsomes but stimulated these activities. This result shows that different species of cytochrome P-450 other than cytochrome P-450C21 catalyzes the 17 alpha-hydroxylation and C17,20 bond cleavage. The stimulation of 17 alpha...Continue Reading

Citations

May 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·D C KossorH D Colby
Sep 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·D XuH D Colby
Mar 24, 2004·Fertility and Sterility·Anne BachelotPhilippe Touraine
Jun 9, 1998·The Journal of Steroid Biochemistry and Molecular Biology·Y HuangH D Colby
Dec 3, 1999·The British Journal of Dermatology·P Y VenencieM Misrahi
Sep 8, 2011·European Journal of Endocrinology·Julie SarfatiUNKNOWN Study Group Hyperandrogenism in Postmenopausal Women
May 13, 2014·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Fumiko Mitani
Oct 18, 2003·Endocrinology·Wataru KishimotoYoshihiko Funae
Oct 16, 2001·The Journal of Clinical Endocrinology and Metabolism·B CouzinetG Schaison
Nov 27, 1987·Biochimica Et Biophysica Acta·S KominamiS Takemori
Jan 2, 1990·Molecular and Cellular Endocrinology·M L Chouinard, H R Fevold
Jul 30, 1991·Biochimica Et Biophysica Acta·A HiguchiS Takemori

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