PMID: 3768892Aug 15, 1986Paper

Immunochemical studies on the combining site of the blood group A-specific lima bean lectin

Carbohydrate Research
S K SikderI J Goldstein

Abstract

The combining site of the lima bean (Phaseolus lunatus) lectin (LBL) was studied by quantitative precipitin and precipitin-inhibition assays. The lectin precipitated best with hog gastric mucosa and human ovarian cyst blood group A1 substances and moderately with A2 substances. B substances precipitated very poorly and H, Lea, Leb, and precursor I substances did not react. Blood group A1 and A2 substances reacted to varying extents and these differences are attributable to heterogeneity resulting from incomplete biosynthesis of carbohydrate chains. By inhibition of precipitation of LBL with A1 blood group substance, the lectin was found to be most specific for fucose-containing oligosaccharides having the A trisaccharide, DGalNAc alpha 1----3[L-Fuc alpha 1----2]DGal determinant. The best inhibitor, an A-specific hexasaccharide, DGalNAc alpha 1----3[LFuc alpha 1----2]DGal beta 1----3DGlcNAc beta 1----3-DGal beta 1----4DGlc, was 11 times more active than the A trisaccharide. A difucosyl oligosaccharide with a second fucose linked alpha 1----3 to the DGlcNAc is less active; fucose linked alpha 1----4 to DGlcNAc was completely inactive. These results suggest that specific interactions with the subterminal sugars may be important in...Continue Reading

References

Jan 1, 1978·Advances in Carbohydrate Chemistry and Biochemistry·I J Goldstein, C E Hayes
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Citations

Jan 21, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Edurne AlonsoFrancisco Hernández
Jan 15, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Galder ValbuenaFrancisco José Sáez

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