PMID: 60233Jun 15, 1976Paper

Immunochemical study of the peptidoglycan of gram-negative bacteria

European Journal of Biochemistry
H Nguyen-HuyC G Wermuth

Abstract

The specificity of antibodies directed against the peptidoglycan of gram-negative bacteria was studied. The peptidoglycans of Proteus vulgaris, Escherichia coli, Moraxella glucidolytica, Neisseria perflava, give identical precipitin reactions. By means of inhibition studies with various peptidoglycan subunits and synthetic peptides, it was shown that the antibodies are essentially directed against the peptide moiety of the peptidoglycan: L-Ala-D-Glu (L)-mesoA2pm-(L)-D-Ala, that the peptide reacts better with antibodies when it is not cross-linked, and that the C-terminal portion-meso-A2pm-D-Ala of the peptide is immunodominant. These results explain the immunological identity of the peptidoglycans of gram-negative bacteria, which possess the same peptide subunit. Only weak cross-reactivity was observed with the peptidoglycans of gram-positive bacteria (Streptococcus faecium, Micrococcus lysodeikticus, Corynebacterium poinsettiae) where meso-diaminopimelic acid is replaced by L-lysine or L-homoserine. However, the peptidoglycan of Bacillus megaterium which possesses the same peptide subunit as gram-negative bacteria, gives only a reaction of partial identity with these bacteria. This result suggests the presence on the peptidogl...Continue Reading

References

Jan 1, 1972·Archiv für Mikrobiologie·H H MartinH J Preusser
Feb 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·E M ABDULLA, J H SCHWAB
Mar 1, 1965·Biochimica Et Biophysica Acta·I TAKEBE

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Citations

Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R ArnonL Chedid
Oct 1, 1984·Annals of the Rheumatic Diseases·H ParkJ T Rosenbaum
Apr 1, 1984·Medicinal Research Reviews·A Adam, E Lederer
Feb 22, 2017·Frontiers in Cellular and Infection Microbiology·Rudian ZhangGong Cheng
Sep 1, 1996·Infection and Immunity·K J BiberstineR S Rosenthal
Mar 1, 1987·Journal of Clinical Microbiology·H I Wergeland, C Endresen

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Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

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