PMID: 804441Apr 1, 1975

Immunochemistry of purified polysaccharide type antigens of group B streptococcal types Ia, Ib, and Ic

Infection and Immunity
H W Wilkinson

Abstract

The HCl-extracted purified polysaccharide type antigens of group B Streptococcus types Ia and Ib were composed of galactose and N-acetylglucosamine in a molar ratio of 3:1 for the Ia antigen and 2:1 for the Ib antigen. Immunochemical data were the same for the Ia antigens of type Ia, purified in this study, and type Ic, purified earlier. Glucosamine inhibited the Ib quantitative precipitin reactions more effectively than did N-acetylglucosamine, whereas the reverse was true of the Ia reaction. Ouchterlony studies were consistent with these observations and also revealed two type-specific precipitin bands with the HCl-extracted Ia antigens. All saline-extracted type antigens, however, formed single Ouchterlony bands that were only partially identical to the corresponding HCl antigens. Purification of the saline antigens was accomplished by treatment with cold trichloroacetic acid and by fractional ethanol precipitation. Immunoelectrophoresis experiments showed that the saline antigens were more negatively charged than the HCl antigens. The presence of sialic acid in the saline antigens probably accounted for their net negative charge and the fact that they were partially degraded by mild acid hydrolysis. The same serological spe...Continue Reading

Citations

Aug 1, 1981·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·L Bevanger, J Iversen
Apr 1, 1979·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·N E Jensen
Feb 1, 1979·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·L Bevanger, J A Maeland

Related Concepts

Process of Absorption
Antigens, Bacterial
Thin Layer Chromatography
Galactosidase
Immunodiffusion Measurement
Haptens
Immunoelectrophoresis
Leftose
Polysaccharides, Bacterial
Precipitin Tests

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