PMID: 6985605Jan 1, 1980Paper

Immunochemistry of Salmonella O-antigens: specificity of rabbit antibodies against the O-antigen 2 determinant elicited by whole bacteria and 3-O-alpha-paratopyranosyl-D-mannopyranosyl conjugated to bovine serum albumin

International Archives of Allergy and Applied Immunology
H JörbeckA A Lindberg

Abstract

The binding specificities of antibodies directed against the Salmonella sero-group A-specific O-antigen 2 determinant were characterized by precipitation-inhibition and enzyme-linked immunosorbent assay inhibition tests. Two different antigen O-2-specific antisera were investigated: one conventional factor O-2 serum (elicited by whole heat-killed Salmonella paratyphi A bacteria) and another elicited by the synthetic disaccharide 3-O-a-paratopyranosyl-D-mannopyranosyl (formula: see text) covalently linked via a p-isothiocyanatophenyl aglycon to bovine serum albumin (PM-BSA). The inhibition data showed that factor O-2 antibodies have combining sites which recognize structures larger than the (formula: see text) disaccharide and equal to or smaller than an O-antigen O-2-specific octasaccharide derived from the S. paratyphi A O-polysaccharide. Although the factor O-2 serum exhibited a high specificity for the homologous S. paratyphi A O-antigen it still precipitated, though weakly, a heterologous Salmonella typhimurium O-antigen. In contrast, anti-PM-BSA antibodies were exclusively specific for the O-2 determinant of the native polysaccharide antigen. The combining sites of these antibodies best recognized the (formula: see text) d...Continue Reading

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.