Immunochromatography test for rapid diagnosis of Mycoplasma pneumoniae infection

Pediatrics International : Official Journal of the Japan Pediatric Society
Shigeru OnariHiroyuki Tsutsumi

Abstract

The sensitivity and specificity of a new rapid Mycoplasma pneumoniae antigen immunochromatography (IC) test, DK-MP-001, were determined using particle agglutination (PA) antibody response and loop-mediated isothermal amplification (LAMP) gene detection as the gold standard. Of 165 patients, 59 were diagnosed with M. pneumoniae infection based on a ≥fourfold rise of serum PA antibody during the course of the illness. Of the first visit swabs, 60 were positive for M. pneumoniae on LAMP, and 49 were positive for M. pneumoniae antigen on IC test. Compared with PA antibody and LAMP, the sensitivity/specificity of the IC test were 81.4% (48/59) and 99.1% (105/106); and 81.7% (49/60) and 100% (105/105), respectively. IC test detected antigen in pharyngeal swabs more sensitively than in nasal swabs for the same subjects (P < 0.05). The IC test performs well enough to be used with pharyngeal swabs at the first examination.

References

Feb 17, 2005·Journal of Medical Microbiology·Riitta RätyMarjaana Kleemola
Mar 17, 2005·Journal of Medical Microbiology·Naoyuki MiyashitaMikio Oka
Mar 3, 2007·American Journal of Respiratory and Critical Care Medicine·Forest W ArnoldUNKNOWN Community-Acquired Pneumonia Organization (CAPO) Investigators
Mar 31, 2015·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Naoyuki MiyashitaNiro Okimoto
Apr 12, 2016·Frontiers in Microbiology·Katherine Loens, Margareta Ieven

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