Immunocompetent truncated E2 glycoprotein of bovine viral diarrhea virus (BVDV) expressed in Nicotiana tabacum plants: a candidate antigen for new generation of veterinary vaccines

Vaccine
Guillermo NelsonMaría Alejandra Alvarez

Abstract

The bovine viral diarrhea virus (BVDV) is the etiological agent responsible for a wide spectrum of clinical diseases in cattle. The glycoprotein E2 is the major envelope protein of this virus and the strongest inductor of the immune response. There are several available commercial vaccines against bovine viral diarrhea (BVD), which show irregular performances. Here, we report the use of tobacco plants as an alternative productive platform for the expression of the truncated version of E2 glycoprotein (tE2) from the BVDV. The tE2 sequence, lacking the transmembrane domain, was cloned into the pK7WG2 Agrobacterium binary vector. The construct also carried the 2S2 Arabidopsis thaliana signal for directing the protein into the plant secretory pathway, the Kozak sequence, an hexa-histidine tag to facilitate protein purification and the KDEL endoplasmic reticulum retention signal. The resulting plasmid (pK-2S2-tE2-His-KDEL) was introduced into Agrobacterium tumefaciens strain EHA101 by electroporation. The transformed A. tumefaciens was then used to express tE2 in leaves of Nicotiana tabacum plants. Western blot and ELISA using specific monoclonal antibodies confirmed the presence of the recombinant tE2 protein in plant extracts. An ...Continue Reading

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Citations

Jun 26, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Yugang GaoLianxue Zhang
Jan 8, 2013·Veterinary Immunology and Immunopathology·María Sol Peréz AguirreburualdeAndrés Wigdorovitz
Aug 8, 2015·Plant Biotechnology Journal·Vanesa RuizAndrés Wigdorovitz
May 8, 2015·Virology Journal·Yugang GaoLianxue Zhang
Dec 4, 2014·Veterinary Microbiology·Yifei LangHuiyun Chang
Jul 22, 2016·Plant Biotechnology Journal·Naila Shahid, Henry Daniell
Oct 19, 2016·International Journal of Molecular Sciences·Young Hee JoungHyun-Soon Kim

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