Immunocytochemical analysis of the expression of gap junction protein connexin 43 in the rat ovary

Molecular Reproduction and Development
A Mayerhofer, R E Garfield

Abstract

The present immunocytochemical study examines in the rat ovary the pattern of expression of connexin 43 (Cx43), a subunit of gap junctions. Using a well-characterized specific antiserum against rat Cx43, immunoreactivity was not detected in the fetal ovary, i.e., prior to follicular formation. However, in the ovary of 20-day-old, 35-day-old, and adult rats, strong Cx43-immunoreactivity was associated with the cell borders of the follicular epithelium/granulosa cells of all developmental stages (primordial follicles, preantral and antral secondary follicles). In general, immunoreactivity of the granulosa cells of large antral follicles appeared more intense than the one of smaller follicles. Staining was also seen in oocytes (cytoplasmic staining). Theca cells of large antral follicles, but not of small follicles were immunoreactive. Immunoreactive interstitial cells were not seen in ovaries of 20- and 35-day-old animals, but staining in these cells was present in adult rats. In large follicles with signs of atresia, granulosa cells lacked Cx43-immunoreactivity, whereas Cx43-immunoreactivity in their theca interna strikingly increased. Corpora lutea in the cyclic adult rats were heterogeneously stained, with either no detectable...Continue Reading

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Citations

Jul 1, 1997·Molecular Reproduction and Development·I Granot, N Dekel
Feb 5, 2003·Biology of the Cell·Véronique Serre-BeinierPaolo Meda
Nov 1, 1996·Zygote : the Biology of Gametes and Early Embryos·G A DissenS R Ojeda
Jul 22, 2005·The Journal of Reproduction and Development·Jun Babaan FeranilToshihiko Nakao
Feb 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Disha PantAnna T Grazul-Bilska
Apr 15, 2006·Cell Communication & Adhesion·Alexander M SimonCharity L Jackson
Mar 12, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·E Martha Pérez-ArmendarizIrma Villalpando
May 14, 2014·Journal of Cellular Biochemistry·Tanya Zappitelli, Jane E Aubin
Dec 20, 2005·Biochimica Et Biophysica Acta·Laetitia MichonPaolo Meda
Oct 18, 2011·Biochimica Et Biophysica Acta·Ilaria PotolicchioPaolo Meda
Jul 11, 2006·Gene Expression Patterns : GEP·Lauri A Willingham-RockyRobert C Burghardt
Mar 1, 2002·Biology of Reproduction·Irit GranotNava Dekel
Oct 21, 2016·Polish Journal of Veterinary Sciences·A J KorzekwaA A Szczepańska
Oct 29, 2015·Journal of Molecular Endocrinology·Wei-Ling FangJiuan-Jiuan Hwang
Apr 20, 1999·Biology of Reproduction·S C JunejaG M Kidder
Oct 21, 2011·Physiological Reviews·Domenico BoscoPaolo Meda
Sep 25, 2003·Physiological Reviews·Juan C SaezEric C Beyer
Jan 10, 2013·American Journal of Physiology. Endocrinology and Metabolism·Huatao ChenMasa-aki Hattori

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