PMID: 8995638Feb 1, 1997Paper

Immunocytochemical analysis of Uukuniemi virus budding compartments: role of the intermediate compartment and the Golgi stack in virus maturation

Journal of Virology
J JänttiE Kuismanen

Abstract

Previous studies have suggested that Uukuniemi virus, a bunyavirus, matures at the membranes of the Golgi complex. In this study we have employed immunocytochemical techniques to analyze in detail the budding compartment(s) of the virus. Electron microscopy of infected BHK-21 cells showed that virus particles are found in the cisternae throughout the Golgi stack. Within the cisternae, the virus particles were located preferentially in the dilated rims. This would suggest that virus budding may begin at or before the cis Golgi membranes. The virus budding compartment was studied further by immunoelectron microscopy with a pre-Golgi intermediate compartment marker, p58, and a Golgi stack marker protein, mannosidase II (ManII). Virus particles and budding virus were detected in ManII-positive Golgi stack membranes and, interestingly, in both juxtanuclear and peripheral p58-positive elements of the intermediate compartment. In cells incubated at 15 degrees C the nucleocapsid and virus envelope proteins were seen to accumulate in the intermediate compartment. Immunoelectron microscopy demonstrated that at 15 degrees C the nucleocapsid is associated with membranes that show a characteristic distribution and tubulo-vesicular morpholog...Continue Reading

References

Oct 1, 1992·Seminars in Cell Biology·G Griffiths, P Rottier
Mar 6, 1992·Cell·I Mellman, K Simons
Jan 30, 1992·Nature·J E Rothman, L Orci
Mar 1, 1991·Journal of Virology·S Y ChenR W Compans
Nov 1, 1991·Journal of Virology·S Y Chen, R W Compans
Jan 1, 1991·The Journal of Cell Biology·R Persson, R F Pettersson
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C E MachamerM G Farquhar
Jan 1, 1989·Methods in Cell Biology·E Kuismanen, J Saraste
Nov 1, 1987·The Journal of Cell Biology·J SarasteM G Farquhar
Sep 1, 1985·Journal of Virology·E KuismanenR F Pettersson
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Dec 1, 1973·Virology·R Pettersson, L Kääriäinen
Aug 1, 1982·Virology·J F Smith, D Y Pifat
Nov 1, 1982·Molecular and Cellular Biology·E KuismanenR F Pettersson
Feb 1, 1982·Journal of Virology·M PesonenR F Pettersson
Nov 3, 1994·Nature·J E Rothman
Jul 1, 1993·The Journal of Cell Biology·A VelascoM G Farquhar
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Mizuno, S J Singer
Mar 1, 1993·Trends in Cell Biology·J Lippincott-Schwartz

❮ Previous
Next ❯

Citations

Aug 17, 2005·Journal of Virology·Reyes R NovoaCristina Risco
Feb 14, 2002·The Journal of General Virology·Tatsuya NagataDick Peters
Mar 9, 2018·Critical Reviews in Microbiology·Yeping SunWenjun Liu
Dec 28, 2002·Journal of Virology·Iñigo J SalanuevaCristina Risco
Apr 9, 2010·Clinical Microbiology Reviews·Colleen B JonssonOlli Vapalahti
Jul 28, 2016·Viruses·Martin SpiegelStefan Pöhlmann
Oct 23, 2010·Cellular Microbiology·Noelia López-Montero, Cristina Risco
Sep 11, 1999·Journal of Virology·I J SalanuevaC Risco
Aug 24, 2019·Frontiers in Cell and Developmental Biology·Alexander A Mironov, Galina V Beznoussenko
Sep 3, 2018·Histochemistry and Cell Biology·Jaakko Saraste, Michaël Marie
Apr 23, 2003·Virology·C F SpiropoulouR W Compans
Aug 29, 2021·Viruses·Kristina MeierMaria Rosenthal
Nov 26, 2010·Virology Journal·Jiandong LiDexin Li
Jul 14, 2010·Virus Research·Nicolás Cifuentes-MuñozNicole D Tischler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.