Immunocytochemical and immunogold analyses of histone H4 acetylation during Chara vulgaris spermiogenesis

Micron : the International Research and Review Journal for Microscopy
Agnieszka Wojtczak

Abstract

Histone acetylation is one of the epigenetic modifications which play a significant role in chromatin remodeling during spermiogenesis. Acetylation of the histone H4 makes the exchange of nucleoproteins easy. Research on mouse spermatogenesis showed that H4 histone acetylated at Lys 12 (H4K12ac) was specific only to spermatids. Immunocytochemical studies of Chara vulgaris spermatids with the use of antibodies against the histone H4K12ac revealed positive reactions in spermatid nuclei at stages I-VII. This reaction, connected with nuclear condensation, was much stronger at the early stages of spermiogenesis than later on. Moreover, it showed that at the stages V-VII in spermatid nuclei the presence of the histone H4K12ac corresponded with DNA double-strand breaks. Electron microscopy studies with the use of immunogold technique revealed an almost twofold difference between the mean total numbers of gold grains in the examined chromatin in both stages. This study showed nearly equal distribution of gold grains on condensed and non-condensed chromatin of spermatids at the stage III/IV (48.11% and 51.89%, respectively). In the later stage-VI, when chromatin condensation proceeded, labeling of condensed chromatin reached 57.27%, whi...Continue Reading

References

Mar 1, 1992·Molecular Reproduction and Development·M L MeistrichC D Allis
May 11, 1990·Nucleic Acids Research·R OlivaG H Dixon
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bendayan, M Zollinger
Nov 1, 2000·Cell·P CheungP Sassone-Corsi
Feb 15, 2001·Trends in Plant Science·A LusserP Loidl
Jul 16, 2003·Molecular and Cellular Biology·Christophe Pivot-PajotSaadi Khochbin
Aug 20, 2004·European Journal of Biochemistry·Jérôme GovinSaadi Khochbin
Mar 18, 2005·Biology of Reproduction·Rémi-Martin Laberge, Guylain Boissonneault
May 2, 2007·Journal of Cellular Biochemistry·Kathryn KurtzManel Chiva
Mar 28, 2008·BMC Cell Biology·David RobertsonClare M Isacke
Jul 11, 2008·Developmental Biology·Mahalakshmi NairXing Dai
Jan 10, 2009·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Kathryn KurtzManel Chiva
Apr 7, 2009·Current Opinion in Genetics & Development·Andrew BassettAndrew Travers
Mar 5, 2011·Biochimica Et Biophysica Acta·Jakob H Waterborg
May 5, 2011·Mutation Research·Singh RajenderAshok Agarwal
Mar 1, 2012·Biochimica Et Biophysica Acta·Sabrina Z JanTinke L Vormer
Jul 31, 2012·Molecular Cell·Dan Feng, Mitchell A Lazar
Aug 31, 2013·Plant Science : an International Journal of Experimental Plant Biology·Misook Ha
Jan 7, 2014·Biochimica Et Biophysica Acta·Chunbin Zou, Rama K Mallampalli
Mar 4, 2014·Biochimica Et Biophysica Acta·Sarah G Swygert, Craig L Peterson
Jul 22, 2014·Micron : the International Research and Review Journal for Microscopy·Wojtczak Agnieszka
Jun 3, 2013·Reproductive System & Sexual Disorders : Current Research·Aly Pang, Om Rennert
Feb 26, 2015·Nucleic Acids Research·Valentina Turinetto, Claudia Giachino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.