PMID: 6172523Jan 1, 1982Paper

Immunocytochemical and ultrastructural localization of keratin polypeptides in normal epidermal and mucosal cells and tissues

The Journal of Investigative Dermatology
T LoeningJ Thivolet

Abstract

Previous studies revealed that the filaments of cytokeratin express different antigenic sites in the basal and suprabasal cell compartment of the skin and oral mucosa. It has been demonstrated that the keratin polypeptide subunit of molecular weight 67,000 dalton (67K) is a valuable marker of the suprabasal cells, since the normal basal cells remain unlabeled by antibodies against this large polypeptide. In the present study, the distribution of the 67K polypeptide has been investigated on human and guinea pig keratinocyte suspensions, purified basal cells of guinea pigs, normal human abdominal skin and normal human buccal mucosa, using the indirect immunoperoxidase method. The results of this investigation confirmed ultrastructurally that the 67K-antigen expression is lacking in the epidermal basal cell compartment. However, it could be shown that 67K polypeptide is a component of the tonofilaments in the upper Malpighian cell layers of the skin and oral mucosa. These observations suggest that keratinocyte differentiation is accompanied by modifications of antigenic determinants of the keratin filaments.

References

Oct 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R H GrayI A Bernstein
Jun 1, 1979·The Journal of General Virology·M J StaquetJ Thivolet
Mar 31, 1979·Archives of Dermatological Research·M Prunieras
Jan 1, 1979·Archives of Oral Biology·L K OberleJ S Greenspan
Aug 1, 1978·The Journal of Investigative Dermatology·H P Baden, L D Lee
Oct 1, 1976·The Journal of Investigative Dermatology·L D LeeB F Fleming
Nov 1, 1976·The Journal of Investigative Dermatology·H P BadenJ Kubilus
Jul 1, 1975·The Journal of Investigative Dermatology·A S Breathnach
Nov 1, 1974·The Journal of Cell Biology·C J Skerrow, A G Matoltsy
Jan 1, 1980·Archives of Dermatological Research·J ViacC Goujon
Feb 1, 1962·The Journal of Investigative Dermatology·A S ZELICKSON, J F HARTMANN

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Citations

Dec 1, 1984·Journal of Oral Pathology·P ReichartJ Becker
Jan 1, 1983·Virchows Archiv. A, Pathological Anatomy and Histopathology·T LöningM vom Dahl
Apr 1, 1988·Journal of Oral Pathology·Z GaoE B Lane
Feb 1, 1983·Journal of Cutaneous Pathology·O P Clausen
Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·D BroekaertE Gillis

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