Immunocytochemical characterization of explant cultures of human prostatic stromal cells

The Prostate
A KooistraF H Schröder

Abstract

The study of stromal-epithelial interactions greatly depends on the ability to culture both cell types separately, in order to permit analysis of their interactions under defined conditions in reconstitution experiments. Here we report the establishment of explant cultures of human prostatic stromal cells and their immunocytochemical characterization. As determined by antibodies to keratin and prostate specific acid phosphatase, only small numbers (< 5%) of epithelial cells were present in primary cultures; subsequent passaging further reduced epithelial cell contamination. Antibodies against intermediate filament proteins (keratins, vimentin, and desmin) and smooth muscle actin microfilaments demonstrated that stromal cells from benign prostatic hyperplasia and prostate carcinoma differed in regard to their differentiation markers. Two contrasting phenotypes were identified in cultures derived from these two different lesions: One exhibiting fibroblastic features, was predominant in cultures derived from benign lesions and a second, showing varying degrees of smooth muscle differentiation, was more abundant in carcinoma-derived cultures. These findings are indicative of a remarkable divergence in the stromal-epithelial relatio...Continue Reading

References

Mar 15, 1978·International Journal of Cancer. Journal International Du Cancer·K R StoneD F Paulson
Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A Schmitt-Gräff, G Gabbiani
Mar 1, 1992·Kidney International·G Gabbiani
Apr 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L Rønnov-JessenO W Petersen
Jul 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J A Ruizeveld de WinterT H van der Kwast
Jan 1, 1990·Virchows Archiv. A, Pathological Anatomy and Histopathology·T HasegawaK Hizawa
Feb 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D M Peehl, T A Stamey
Jan 1, 1987·Virchows Archiv. A, Pathological Anatomy and Histopathology·S C Henzen-LogmansC J Meijer
Mar 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·O W Petersen, B Van Deurs
Nov 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C RicciardelliW D Tilley
Mar 1, 1988·Journal of Steroid Biochemistry·J Orlowski, A F Clark
Aug 1, 1987·Endocrine Reviews·G R CunhaY Sugimura
Jun 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D M PeehlT A Stamey
Sep 1, 1985·Cell Differentiation·G R CunhaY Sugimura
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·M OsbornK Weber
Jun 1, 1981·Human Pathology·T A SeemayerR Lagacé
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·W W FrankeK Illmensee

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Citations

Oct 12, 2000·The Prostate·D M Peehl, R G Sellers
May 25, 2004·British Journal of Pharmacology·Dinesh SrinivasanAnindya Bhattacharya
Nov 16, 2004·European Journal of Pharmacology·Dinesh SrinivasanAnindya Bhattacharya

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