Immunocytochemical demonstration of pituitary adenylate cyclase activating polypeptide (PACAP) in the porcine epiphyseal cartilage canals

Neuropeptides
H H Strange-VognsenJ Hannibal

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP), a member of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon family, is known to be a powerful stimulator of adenylate cyclase. Recently, PACAP has been shown to stimulate cAMP in osteoblast-like cells and mouse calvarian bones. In the present study, PACAP immunoreactivity (IR) was demonstrated in cartilage canals from newborn and 3-4-week-old pigs. In tissues from the femoral head and the patella with and without ossification centres, PACAP-IR nerve fibres were found in the cartilage canals innervating blood vessels. The pattern of distribution was not dependent on age or the occurrence of an ossification centre. Co-localization studies showed a high degree of co-localization with calcitonin gene-related peptide (CGRP) and substance P (SP) but little co-localization with VIP. Our findings support earlier findings of CGRP, SP and VIP in bone tissue and add PACAP to the group of neuropeptides with a sensory and/or modulatory function in bone tissue.

References

Jan 1, 1991·International Orthopaedics·A Bjurholm
Oct 16, 1989·Biochemical and Biophysical Research Communications·A MiyataD H Coy
May 1, 1972·The Anatomical Record·N J Wilsman, D C Van Sickle
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J R Pisegna, S A Wank
Oct 1, 1995·The Anatomical Record·G SisaskA Kreicbergs

❮ Previous
Next ❯

Citations

Aug 31, 2002·Microscopy Research and Technique·Pernilla Lundberg, Ulf H Lerner
Jul 29, 2006·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S-D JiangL-Y Dai
Jun 27, 2013·Cellular and Molecular Neurobiology·Sayako NonakaTeruko Takano-Yamamoto
May 23, 2003·Journal of Neurophysiology·Colleen C HeggMary T Lucero
Aug 13, 2014·Journal of Molecular Neuroscience : MN·Tamás JuhászRóza Zákány
Feb 7, 2016·Bone·Cecília J AlvesMeriem Lamghari
Apr 9, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·M Horvat-GordonR M Leach
Jun 15, 2016·Medicinal Research Reviews·Zaid AmsoMargaret A Brimble
Sep 6, 2005·Clinics in Plastic Surgery·Birgit Weyand, Herbert P von Schroeder
Feb 24, 2015·Peptides·Tamás JuhászRóza Zákány
May 16, 2006·Osteoarthritis and Cartilage·M A KarsdalC Christiansen
Jan 27, 2018·International Journal of Molecular Sciences·Susanne Grässel, Dominique Muschter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.