Immunocytochemical detection and cytomorphology of lymphocyte subpopulations in a teleost fish Dicentrarchus labrax

Cell and Tissue Research
N RomanoG Scapigliati

Abstract

The monoclonal antibodies DLT15 and DLIg3 directed against thymocytes and serum immunoglobulins of the sea bass (Dicentrarchus labrax, L.) were used to study cells from the thymus, head kidney, spleen, gut-associated lymphoid tissue and peripheral blood leukocytes of this fish by immunofluorescence and pre-embedding immunoelectron microscopy. Immunofluorescence and flow cytometry of leukocyte fractions revealed a large number of DLT15-positive cells in the thymus (approximately 80%) and intestine (approximately 55%) and fewer cells in the spleen (approximately 7%), head kidney (approximately 6%) and peripheral blood (approximately 3%). DLT15-positive cells had two main morphologies, both detectable among thymocytes: a large round heterochromatic nucleus with light sparse cytoplasm (type a) and an irregular and heterocromatic nucleus with cytoplasm rich in polysomes and mitochondria (type b). Type b was most represented in spleen, head kidney, intestine and blood. We suggest that the type b morphology represents more differentiated lymphocytes. Flow cytometry revealed numerous DLIg3-positive cells in the head kidney (approximately 33%), spleen (approximately 30%) and peripheral blood leukocytes (approximately 21%) and fewer posi...Continue Reading

Citations

Nov 7, 2001·Developmental and Comparative Immunology·G ScapigliatiL Abelli
Aug 11, 2001·Veterinary Immunology and Immunopathology·L Petrie-Hanson, A J Ainsworth
Apr 17, 2012·Developmental and Comparative Immunology·Catia MarozziGiuseppe Scapigliati
Dec 3, 2011·Developmental and Comparative Immunology·Irene SalinasJ Oriol Sunyer
Jan 31, 2009·Veterinary Immunology and Immunopathology·Shou-Ming FengXiu-Ting Qiao
Aug 30, 2008·Fish & Shellfish Immunology·Elisa RandelliGiuseppe Scapigliati
Mar 27, 2007·Veterinary Immunology and Immunopathology·Dimitry A ChistiakovF A M Volckaert
Aug 12, 2000·Fish & Shellfish Immunology·G ScapigliatiC J Secombes
Apr 12, 2003·Fish & Shellfish Immunology·Eva JanssonLars Pilström
Nov 18, 2000·Fish & Shellfish Immunology·N M dos SantosJ H Rombout
Aug 3, 2010·Journal of Immunological Methods·Gyri Teien HauglandHeidrun I Wergeland
May 3, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P DomeniciA Shingles

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