PMID: 9181694Jun 15, 1997Paper

Immunocytochemical detection and spatial distribution of myosin light-chain kinase in preimplantation mouse embryos

The Journal of Experimental Zoology
N KabirH Hidaka

Abstract

As a follow-up to our previous study on the role of myosin light-chain kinase (MLCK), a Ca2+/calmodulin-dependent enzyme, in the development of preimplantation mouse embryos, we examined the presence and pattern of distribution of MLCK during preimplantation development of the mouse by whole-mount, indirect immunocytochemistry and by Western blotting, using a monoclonal antibody against MLCK. At all stages of preimplantation development, the nucleus was brightly stained with an unstained region around the nucleus, and regions near the cell membrane were also brightly stained. Using the optical sectioning capability of the confocal laser scanning microscope, we found that, up to the eight-cell stage, the regions of cell contact were mostly unstained, but along with the process of compaction, cell contact regions showed a clear staining pattern along with clearing of the cytoplasm. During formation of the blastocyst, a ring of immunofluorescence was found at the margin of the blastocoel. In the blastocyst, cells of the inner cell mass were less immunofluorescent than trophectoderm cells. These staining results appear to be due to specific immunoreaction between MLCK and the antibody, because the staining patterns were abolished w...Continue Reading

References

Jun 1, 1978·Experimental Cell Research·S L Abreu, R L Brinster
Jun 1, 1992·Brain Research. Molecular Brain Research·A M EdelmanJ Cho-Lee
May 1, 1992·Differentiation; Research in Biological Diversity·H G SlagerC L Mummery
Jul 1, 1990·Molecular Reproduction and Development·T Bloom, J McConnell
Aug 1, 1988·Cell Differentiation·E LehtonenI Reima
Oct 1, 1988·The Journal of Experimental Zoology·H Yamaura, A Spindle
Aug 1, 1985·The Journal of Experimental Zoology·A SpindleR A Pedersen
Sep 1, 1984·The Journal of Cell Biology·J S Sobel
Jan 1, 1983·Developmental Biology·J S Sobel
Jul 1, 1984·Journal of Reproduction and Fertility·P L Pakrasi, S K Dey
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P de LanerolleK Burridge

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Citations

Jun 5, 2004·Biochemical and Biophysical Research Communications·Rikako KawagishiYuji Murata
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·N L StephensX Ma

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