Immunocytochemical diagnosis of early myocardial ischaemic/hypoxic damage

Forensic Science International : Synergy
S LeadbeatterB Jasani

Abstract

The sensitive and reliable dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure (B. Jasani et al., Virchows Arch (Pathol. Anat.), 406 (1985) 441-448) was used to study the distribution of immunoperoxidase staining seen with antibodies to seven protein markers in post-mortem heart tissue. This was obtained from 12 cases with macroscopic myocardial infarction and 17 cases without myocardial infarction (10 with and 7 without significant coronary artery atherosclerosis). The immunostaining patterns were compared with the appearances seen in adjacent sections stained by the routine haematoxylin and eosin (H & E) and phosphotungstic acid haematoxylin (PTAH) methods and a method previously recommended for the detection of early myocardial infarction, the haematoxylin basic fuchsin picric acid (HBFP) stain. Loss of immunostaining with an antibody to myoglobin was found to be a reliable and more objective marker of both early and established myocardial infarction compared with the histological stains. Antibodies to myosin, caeruloplasmin, C-reactive protein and pre-albumin gave similar but less reliable results, whilst those to complement factor C3b and alpha-1 anti-trypsin gave the least reliable results for early myocardial i...Continue Reading

References

Jul 1, 1976·Forensic Science·B Knight
May 1, 1979·Forensic Science International : Synergy·B Knight
May 1, 1987·Forensic Science International : Synergy·B Badir, B Knight
Mar 1, 1987·The American Journal of Forensic Medicine and Pathology·S B Karch
Aug 1, 1987·Journal of Clinical Pathology·G G Fechner, S Sivaloganathan
Jan 1, 1985·Virchows Archiv. A, Pathological Anatomy and Histopathology·B JasaniA R Gibbs
Mar 1, 1985·Annals of Clinical Biochemistry·J M McCombA A Jadgey
Dec 1, 1969·The American Journal of Cardiology·R B Jennings
Jan 1, 1982·Annals of the New York Academy of Sciences·I M GoldsteinG Weissmann
Nov 1, 1984·The American Journal of Cardiology·H A KatusB A Khaw
Jan 1, 1983·British Heart Journal·H GilutzM R Quastel
Nov 1, 1980·The Journal of Clinical Investigation·R N PinckardM S Olson

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Citations

Jan 1, 1991·International Journal of Legal Medicine·G FechnerB Brinkmann
Apr 23, 1996·Forensic Science International : Synergy·B J HuJ Z Zhu
Jul 12, 1996·Forensic Science International : Synergy·J M Zhang, L Riddick
Dec 22, 1999·Forensic Science International : Synergy·A FieguthW J Kleemann
Jun 18, 2014·Forensic Science, Medicine, and Pathology·Mustafa Talip SenerAhmet Nezih Kok
Mar 1, 1990·Forensic Science International : Synergy·S LeadbeatterB Jasani
Jul 10, 2016·International Journal of Legal Medicine·Sara SabatassoValentin Djonov
Aug 22, 2009·The American Journal of Forensic Medicine and Pathology·Yi Shao HuaGuang Zhao Huang
Jun 4, 2008·The American Journal of Forensic Medicine and Pathology·Carlo P CampobassoMassimo F Colonna
Apr 16, 2002·The American Journal of Forensic Medicine and Pathology·Alfredo Ribeiro-SilvaMarcos A Rossi
May 13, 2017·International Journal of Legal Medicine·Sara SabatassoTony Fracasso

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