Immunocytochemical differentiation of canine mesenchymal tumors in cytologic imprint preparations

Veterinary Clinical Pathology
R HöinghausReinhard Mischke

Abstract

Immunocytochemical techniques are a potentially valuable diagnostic tool to support cytologic diagnosis in dogs. However, detailed studies of staining patterns and intensity in cytologic specimens of mesenchymal tumor types are lacking. The aim of this study was to evaluate commercially available antibodies against human proteins for use in the characterization of canine tumors of mesenchymal origin in cytologic samples. Immunocytochemical staining was performed on air-dried imprint specimens of biopsies obtained from 103 mesenchymal neoplasms and 14 metastatic lesions from 98 dogs. All specimens were stained with anti-cytokeratin AE1/AE3 and vimentin. Based on the histologic diagnosis, tumors of muscle, endothelial, histiocytic, and melanocytic origin also were stained with cell-specific antibodies. Staining intensity was subjectively graded and the percentage of positive tumor cells was estimated. All mesenchymal tumors and metastases, with the exception of mesotheliomas, were vimentin-positive and cytokeratin-negative; mesotheliomas (n=6) were positive for both vimentin and cytokeratin. Tumors of muscle (n=5), endothelial (n=15), and histiocytic (n=18) origin stained moderately to strongly positive in a majority of tumor cel...Continue Reading

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Citations

Jun 28, 2011·Journal of Feline Medicine and Surgery·Michelle A Morges, Karen Zaks
Apr 28, 2009·Veterinary Clinical Pathology·Anne Barger, Melissa Riensche
Oct 23, 2013·The Veterinary Clinics of North America. Small Animal Practice·Joanne Hodges
May 21, 2011·Topics in Companion Animal Medicine·Amy L MacNeill
Feb 8, 2011·Clinics in Laboratory Medicine·Leslie C Sharkey, Maxey L Wellman
Apr 16, 2014·Veterinary Clinical Pathology·Brett M Stone, David Gan
May 11, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Adam D AulbachMatti Kiupel
Apr 15, 2014·Polish Journal of Veterinary Sciences·R Przeździecki, R Sapierzyński
Feb 3, 2015·Veterinary Clinical Pathology·Koranda A WallaceReema T Patel

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