Immunocytochemical evidence for the presence of histamine and GABA in photoreceptors of the barnacle (Balanus nubilus)

Visual Neuroscience
J C CallawayJ S Edwards

Abstract

Biochemical evidence indicates that GABA and histamine may both be synthesized by barnacle photoreceptors (Koike & Tsuda, 1980; Timpe & Stuart, 1984; Callaway & Stuart, 1989b). We used antisera against GABA- and histamine-protein conjugates to determine whether the photoreceptors contain either or both of these antigens. Both antisera labeled all of the photoreceptors in each of the three ocelli. Histamine-like immunoreactivity was found throughout each photoreceptor cell but was most intense at their presynaptic terminals. Histamine-like immunoreactivity was blocked by preincubation of the antibody either with histamine or with a histamine-protein conjugate. GABA-like immunoreactivity was found in all parts of the photoreceptors including the cell body, axon, rhabdomeric dendrites, and presynaptic terminals. GABA-protein conjugates blocked the GABA-like labeling of the photoreceptors, while protein conjugates with histamine, L-glutamate, L-glutamine, beta-alanine, and taurine did not. Histamine-like immunoreactivity in the supraesophageal ganglion was confined to the photoreceptor terminals and a second, loose plexus of endings in the main neuropil. GABA-like immunoreactivity, in contrast, was found in approximately twenty-fiv...Continue Reading

References

Jun 2, 1977·Nature·J D Turner, G A Cottrell
Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger
Jun 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I Nenci
Aug 1, 1987·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·R C Hardie
Jun 1, 1987·Cellular and Molecular Neurobiology·J M Saavedra
Dec 15, 1988·The Journal of Comparative Neurology·G A EngbretsonJ Y Wu
Mar 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P PanulaA Virkamäki
Jan 1, 1986·Cell and Tissue Research·S G HoskinsJ G Hildebrand
Nov 1, 1986·Journal of Neurochemistry·R IentileS Macaione
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·E A KravitzZ W Hall
Jun 1, 1970·The Journal of Physiology·H M BrownR M Meech
Jul 30, 1984·Brain Research·L C Timpe, A E Stuart
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Feb 1, 1981·The Journal of Physiology·D Oertel, A E Stuart
Feb 1, 1983·Journal of Neurophysiology·L A OlandA E Stuart
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P PanulaE Costa
Aug 1, 1983·Journal of Neurochemistry·M S Elias, P D Evans
Apr 22, 1982·Brain Research·C H BaileyI Kupfermann

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Citations

Oct 1, 2013·Neuroscience Letters·Lorenzo GallusMaria Angela Masini
Aug 6, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Barbara-Anne Battelle, Mary K Hart
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·B F Murphy, J L Larimer
May 29, 2007·Progress in Neurobiology·Ann E StuartIan A Meinertzhagen
Mar 20, 1999·Microscopy Research and Technique·J C Callaway, A E Stuart
Aug 24, 2000·The Journal of Comparative Neurology·S MichelP A Stevenson
Nov 1, 1991·Journal of Neurochemistry·P V Sarthy
Mar 22, 1991·The Journal of Comparative Neurology·B A BattelleA E Stuart
Jun 1, 1997·Current Eye Research·Y KusakariM Tamai
May 22, 1991·The Journal of Comparative Neurology·U Homberg, J G Hildebrand

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