PMID: 7024214Jan 1, 1981Paper

Immunocytochemical identification of amyloid in formalin-fixed paraffin sections

Histochemistry
T ShirahamaA S Cohen

Abstract

Formalin-fixed paraffin sections of livers, spleens and kidneys from patients with primary, secondary and familial amyloidosis as well as from a casein-induced murine amyloid model were analysed by an immunocytochemical (unlabeled antibody enzyme) method utilizing antisera to amyloid-related proteins. All amyloid deposits of all amyloid types showed positive reactions with anti-AP of the respective species. Positive reaction of anti-human AA to human secondary amyloid deposits and of anti-mouse AA to the deposits of casein-induced murine amyloid was also observed, but there was no species cross reactivity. No significant deposition of the reaction products was produced by anti-immunoglobulin light chains on deposits of any amyloid type, or by anti-AA in the tissues from primary or familial amyloidosis. The results indicate that amyloid proteins AA and AP can survive as antigens through routine histologic preparation, that anti-AP can be a universal marker for deposits of any amyloid type within the same species, and that AA-type amyloid can be identified by this method while there may as yet be no feasible universal marker for the AL-type at present.

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Citations

Jan 1, 1985·Rheumatology International·D R MitrovicA Ryckewaert
Aug 1, 1987·Journal of Neurology·M YamadaM Hayakawa
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·L Pajor, G Kelényi
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·C A van de KaaE Gruys
Aug 10, 2000·Italian Journal of Neurological Sciences·M P GrassiA Mangoni
Jul 1, 1995·Critical Reviews in Oncology/hematology·E Pascali
Dec 1, 1989·Mayo Clinic Proceedings·M A Gertz, R A Kyle
Dec 1, 1986·Pathology, Research and Practice·P Chastonay, J Hurlimann
Apr 1, 1992·Histopathology·S E Trotter
Apr 1, 1987·Postgraduate Medical Journal·D P O'DohertyK F Wood
Mar 1, 1984·American Journal of Ophthalmology·B H DoftA S Cohen
Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T ShirahamaA S Cohen
Jul 1, 1988·Stain Technology·M T Elghetany, A Saleem
Jul 24, 2012·Progress in Histochemistry and Cytochemistry·Reinhold P Linke
Mar 15, 2006·Annals of the New York Academy of Sciences·Stephen C Meredith
Feb 1, 1983·Experimental Eye Research·G E Wheeler, R A Eiferman
Jan 1, 1982·Annals of the New York Academy of Sciences·M SkinnerA S Cohen
Sep 1, 1985·Scandinavian Journal of Immunology·M A GertzR A Kyle
Aug 1, 1993·Journal of Hepatology·L A OsickG M Comer
Nov 1, 1987·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology·H LöfbergA Grubb
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Jan 1, 1982·Acta Neuropathologica·J M PowersC J Rosenthal
Aug 1, 1985·Journal of the Neurological Sciences·I ElovaaraT Erkinjuntti
Jan 1, 1990·Critical Reviews in Oncology/hematology·R A Kyle, M A Gertz

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