PMID: 9179930May 1, 1997Paper

Immunocytochemical identification of the oestrogen receptor in the nuclei of cultured human placental syncytiotrophoblasts

Placenta
R B BilliarE D Albrecht

Abstract

We have shown that oestrogen has a central integrative role in regulating key components of the progesterone biosynthetic and corticosteroid metabolic pathways within syncytiotrophoblasts that govern placental function and maturation of the fetal pituitary-adrenocortical axis. Studies utilizing classic binding procedures and RNAse protection have demonstrated that human placental villous tissue exhibits specific high affinity oestrogen binding and expresses the mRNA for the oestrogen receptor. However, it is not known whether the oestrogen receptor is expressed specifically in syncytiotrophoblasts. Therefore, the present study determined whether the oestrogen receptor protein was detectable by immunocytochemistry in cultured human syncytiotrophoblast maintained in a low oestrogen/progestin environment. Cytotrophoblasts were isolated from human term placentae by trypsin dispersion and Percoll gradient centrifugation and cultured for 5, 7 or 10 days. Incubation of syncytiotrophoblast with 5-10 micrograms/ml of the anti-oestrogen receptor rat monoclonal antibody D-75, which is specific for the primate oestrogen receptor, resulted in identification of the oestrogen receptor in the nuclei of these cells. In contrast, there was no re...Continue Reading

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Citations

Oct 19, 2013·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Joyce Chai, Pak-Chung Ho
Nov 15, 2007·Stem Cells and Development·Catherine A VandevoortGordon C Douglas
Oct 31, 2009·The International Journal of Developmental Biology·Eugene D Albrecht, Gerald J Pepe
Jun 15, 2011·Molecular and Cellular Endocrinology·Jessie Z-J ChenRosemary J Keogh
Nov 21, 2007·Molecular Reproduction and Development·Eugene D AlbrechtGerald J Pepe
Nov 5, 2011·Molecular and Cellular Endocrinology·Jessie Z-J ChenRosemary J Keogh
Jan 25, 2000·American Journal of Physiology. Cell Physiology·W X WuP W Nathanielsz
Jan 18, 2006·Molecular and Cellular Endocrinology·R YashwanthA Jagannadha Rao

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