PMID: 7019220Jul 1, 1981Paper

Immunocytochemical identification of phenylalanine hydroxylase and albumin in cultured hepatoma cells and isolated rat hepatocytes

The Journal of Cell Biology
R E BakerR Shiman

Abstract

Rhodamine-conjugated antibodies specific for phenylalanine hydroxylase and serum albumin were employed as cytochemical probes to identify these two proteins in H4 hepatoma cells and in isolated rat hepatocytes. Each fluorescent antibody stained the cells specifically and in a distinctive manner. In both cell types, albumin staining was discretely localized in cytoplasmic and in H4 cultures varied somewhat from cell to cell. Evidence from cultures of REB15 cells, a strain derived by cloning H4 cells in tyrosine-free medium, suggested that the staining variability of H4 cells could reflect a variability in phenylalanine hydroxylase content. Hydrocortisone-treated H4 cells and REB15 cultures contain increased amounts of phenylalanine hydroxylase; and all cells in the culture appear to be induced by the hormone. Evidence was presented to show that the albumin visualized within the isolated hepatocytes had been synthesized by these cells, and, furthermore, that quantitatively nearly all intracellular albumin in the isolated rat hepatocytes appeared to be entrained in the secretion pathway (analogous data already exist for H4 cells [Baker, R.E., and R. Shiman. 1979. J. Biol. Chem. 254:9633-9639]). By scoring specific fluorescence, 86...Continue Reading

References

Jun 30, 1975·Annals of the New York Academy of Sciences·P Brandtzaeg
Aug 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M HorikawaT Onoé
Nov 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A GuillouzoJ F Chiu
Nov 1, 1979·Developmental Biology·R N Carlsson, B I Ingvarsson
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S H OrkinP Leder
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M H HeggenessS J Singer
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·C B Laurell
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·E B ThompsonJ F Curran
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Jan 1, 1968·Histochemie. Histochemistry. Histochimie·N Chandrasekharan
Nov 1, 1969·Archives of Biochemistry and Biophysics·M Steinbuch, R Audran
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT
Feb 1, 1964·The Journal of Cell Biology·Y HAMASHIMAA H COONS

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Citations

Feb 12, 1983·Lancet·A S Bulman, E Heyderman
Nov 3, 2004·Analytical Biochemistry·Boontarika BoonyapiwatGlyn B Steventon
May 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Jungermann, N Katz

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