PMID: 9184845Apr 1, 1997Paper

Immunocytochemical identification of metallothionein-positive cells in rheumatoid synovium and analysis of their cell lineage

The Histochemical Journal
C WintersA J Morgan

Abstract

Metallothionein is a ubiquitous low molecular weight metalloprotein with powerful protective properties against oxygen radical-mediated cytotoxicity associated with inflammatory processes. In rheumatoid arthritis, the inflammatory damage to the synovium appears to be mediated by free radicals released by the high concentration of neutrophils found in the synovial fluid of the inflamed joint. Synovial tissue obtained during routine surgery on rheumatoid and non-rheumatoid joints was subjected to an indirect immunoperoxidase protocol for the immunolocalization of metallothionein using mouse monoclonal anti-metallothionein antibody E9, reactive against the two major isoforms of mammalian metallothionein. A layer of large dendritic-like cells situated subsynovially in the rheumatoid synovium stained very positively for the metalloprotein, both cytoplasmically and in their nuclei. These cells were not found in non-rheumatoid osteoarthritic or in undamaged synovial tissue associated with traumatic joint injury. An attempt was made to investigate their lineage using a series of antibody markers against epithelial cells, endothelial cells, smooth muscle, mesothelial cells, fibroblasts, neutrophils, dermal dendrocytes, macrophages, low ...Continue Reading

Citations

Apr 6, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J H BeattieA K West
Jun 22, 2001·The Journal of Biological Chemistry·S R StürzenbaumP Kille
Jun 16, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·E Canpolat, M A Lynes
Feb 28, 2003·Applied Immunohistochemistry & Molecular Morphology : AIMM·P DziegielM Zabel

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