Abstract
Caspase-3 has been recognised as a main effector caspase of the apoptotic cascade. Involvement of caspase-3 has been implicated in a beta-cell cloned cell line from type 1 diabetic subjects and in isolated islets from type 2 diabetic subjects. This study aimed to immunocytochemically identify cleaved caspase-3 positive islet cells in type 2 diabetic subjects compared with control subjects. Using commercially available rabbit anti-cleaved caspase-3 antibody, immunocytochemical staining was performed on 16 cases of pancreatic tissues from type 2 diabetic subjects compared with age-matched controls. Control islets revealed cleaved caspase-3 positive cells in about 4.7% in total islet cells with large and small islets positive at 4.1% and 7.0%, respectively. Islets from type 2 diabetic subjects showed higher immunostaining percentage at 8.7% in total islets with large and small islets positive for cleaved caspase-3 at 7.7% and 12%, respectively, at about twice that of the control values. Islets from type 2 diabetics were generally insulin cell-less and glucagon cell-rich, but insulin cells still remained. Type 2 diabetic islets showed various stromal amyloid deposits, displacing the residual islet cells. Cleaved caspase-3 positive ...Continue Reading
References
Nov 1, 1978·Diabetologia·P Westermark, E Wilander
Aug 11, 1995·Cell·S J Martin, D R Green
Jan 1, 1995·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·G C Viberti
Apr 25, 1996·Nature·M EnariS Nagata
Mar 28, 1998·The Journal of Experimental Medicine·H HirataM Sasada
Mar 17, 1999·Diabetes·K YamadaK Nonaka
Mar 17, 1999·Diabetes·J JansonP C Butler
Aug 10, 2000·The New England Journal of Medicine·J W HöppenerC J Lips
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Allen M Gown, Mark C Willingham
Aug 9, 2002·Pathology International·Tatsuo Tomita
Dec 28, 2002·Diabetes·Alexandra E ButlerPeter C Butler
Sep 26, 2003·Pathology International·Tatsuo Tomita
Mar 1, 2005·Diabetes·Sabina E PerezMatthias Hebrok
Nov 25, 2005·Diabetes·Miriam CnopDecio L Eizirik
Nov 26, 2010·Islets·Tatsuo Tomita
Nov 26, 2010·Islets·Tatsuo Tomita
Citations
Oct 18, 2012·American Journal of Physiology. Endocrinology and Metabolism·Xiaoyong LeiSasanka Ramanadham
Nov 1, 2012·Journal of Translational Medicine·Anita V Neutzsky-WulffMorten A Karsdal
Feb 14, 2016·Journal of Molecular Histology·Ahmed A M Abdel-Hamid, Alaa El-Din L El-Firgany
Sep 10, 2015·Endocrine·Asuka MoritaTakashi Miki
Aug 1, 2012·Islets·Tatsuo Tomita
Oct 11, 2011·Islets·Tatsuo Tomita
Oct 21, 2017·Endocrine Reviews·Amin Ardestani, Kathrin Maedler
Apr 19, 2018·Diabetes/metabolism Research and Reviews·Giuseppina T RussoUNKNOWN BetaDecline Study Group
Nov 5, 2019·Nature Communications·Amin ArdestaniKathrin Maedler
Mar 1, 2017·Expert Review of Endocrinology & Metabolism·Ting YuanAmin Ardestani
Jul 27, 2021·Journal of Internal Medicine·Jiangming SunAndreas Edsfeldt