Immunocytochemical localisation of caspase-3 in pancreatic islets from type 2 diabetic subjects

Pathology
T Tomita

Abstract

Caspase-3 has been recognised as a main effector caspase of the apoptotic cascade. Involvement of caspase-3 has been implicated in a beta-cell cloned cell line from type 1 diabetic subjects and in isolated islets from type 2 diabetic subjects. This study aimed to immunocytochemically identify cleaved caspase-3 positive islet cells in type 2 diabetic subjects compared with control subjects. Using commercially available rabbit anti-cleaved caspase-3 antibody, immunocytochemical staining was performed on 16 cases of pancreatic tissues from type 2 diabetic subjects compared with age-matched controls. Control islets revealed cleaved caspase-3 positive cells in about 4.7% in total islet cells with large and small islets positive at 4.1% and 7.0%, respectively. Islets from type 2 diabetic subjects showed higher immunostaining percentage at 8.7% in total islets with large and small islets positive for cleaved caspase-3 at 7.7% and 12%, respectively, at about twice that of the control values. Islets from type 2 diabetics were generally insulin cell-less and glucagon cell-rich, but insulin cells still remained. Type 2 diabetic islets showed various stromal amyloid deposits, displacing the residual islet cells. Cleaved caspase-3 positive ...Continue Reading

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Citations

Oct 18, 2012·American Journal of Physiology. Endocrinology and Metabolism·Xiaoyong LeiSasanka Ramanadham
Feb 14, 2016·Journal of Molecular Histology·Ahmed A M Abdel-Hamid, Alaa El-Din L El-Firgany
Oct 21, 2017·Endocrine Reviews·Amin Ardestani, Kathrin Maedler
Apr 19, 2018·Diabetes/metabolism Research and Reviews·Giuseppina T RussoUNKNOWN BetaDecline Study Group
Nov 5, 2019·Nature Communications·Amin ArdestaniKathrin Maedler
Mar 1, 2017·Expert Review of Endocrinology & Metabolism·Ting YuanAmin Ardestani
Jul 27, 2021·Journal of Internal Medicine·Jiangming SunAndreas Edsfeldt

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