Oct 1, 1977

Immunocytochemical localization of a folicle stimulating hormone-like molecule in the testis

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J C HutsonG C Moriarty

Abstract

A follicle-stimulating hormone (FSH)-like molecule was localized in normal adult rat testes as well as testosterone-treated hypophysectomized rat tests with an unlabeled antibody (anti-FSH), peroxidase-antiperoxidase complex technique. Anti-FSH bound specifically to ultrathin sections of acrosomes of spermatids and intranuclear bodies of early spermatids. Quantitation of staining intensity demonstrated that FSH, used as an absorbing antigen, would significantly reduce this binding. There was less anti-FSH binding to the acrosomes of spermatozoa in the body and tail of the epididymis as compared to the less mature germ cells located in the testis and head of the epididymis. The acrosomal and nuclear staining of spermatids taken from hypophysectomized animals was similar to staining observed in sham injected animals. Taken together, these results suggest that there is a molecule within the acrosome that is immunologically similar to FSH. Most importantly, these results emphasize the importance of conducting physiologic experiments in conjunction with immunocytochemical studies.

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Mentioned in this Paper

Establishment and Maintenance of Localization
Testosterone Measurement
Benign Neoplasm of Testis
Salicylhydroxamic acid
Neoplasm of Uncertain or Unknown Behavior of Testis
Sperm Cell
Spermatozoa Cell Count
Head of Epididymis
Inferior Colliculus
Testis

About this Paper

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