PMID: 9161841Mar 1, 1997Paper

Immunocytochemical localization of copper,zinc superoxide dismutase in peroxisomes from watermelon (Citrullus vulgaris Schrad.) cotyledons

Free Radical Research
L M SandalioL A del Río

Abstract

In previous works using cell fractionation methods we demonstrated the presence of a Cu,Zn-containing superoxide dismutase in peroxisomes from watermelon cotyledons. In this work, this intracellular localization was evaluated by using western blot and EM immunocytochemical analysis with a polyclonal antibody against peroxisomal Cu,Zn-SOD II from watermelon cotyledons. In crude extracts from 6-day old cotyledons, analysis by western blot showed two cross-reactivity bands which belonged to the isozymes Cu,Zn-SOD I and Cu,Zn-SOD II. In peroxisomes purified by sucrose density-gradient centrifugation only one cross-reactivity band was found in the peroxisomal matrix which corresponded to the isozyme Cu,Zn-SOD II. When SOD activity was assayed in purified peroxisomes two isozymes were detected, Cu,Zn-SOD II in the matrix, and a Mn-SOD in the membrane fraction which was removed by sodium carbonate washing. EM immunocytochemistry of Cu,Zn-SOD on sections of 6-day old cotyledons, showed that gold label was mainly localized over plastids and also in peroxisomes and the cytosol, whereas mitochondria did not label for Cu,Zn-SOD.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J D CrapoL Y Chang
Nov 1, 1992·Free Radical Biology & Medicine·L A del RíoF J Corpas
Sep 15, 1992·The Biochemical Journal·M J de Hoop, G Ab
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G A KellerR A Hallewell
Jan 23, 1992·Biochimica Et Biophysica Acta·R J Wanders, S Denis
Jun 21, 1990·Biochimica Et Biophysica Acta·W NiR N Trelease
May 1, 1989·The Journal of Cell Biology·S J GouldS Subramani
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jun 17, 1994·Annals of the New York Academy of Sciences·I SinghA K Singh
Jan 1, 1993·Annual Review of Cell Biology·S Subramani

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Citations

Nov 19, 2010·Journal of Chemical Ecology·Jacques M Berner, Aimie J Van der Westhuizen
Jan 19, 2000·Clinical Biochemistry·J M MatésI Núñez de Castro
Apr 25, 2001·Physiologia Plantarum·Takashi UshimaruHideo Tsuji
Feb 25, 2003·Journal of Experimental Botany·Luis A del RíoBarbara A Zilinskas
Jan 4, 2012·Journal of Experimental Botany·Ana P Ortega-GalisteoMaría C Romero-Puertas
Aug 12, 2014·Journal of Plant Physiology·Eduardo Lopez-Huertas, Luis A del Río
Sep 22, 2009·Free Radical Biology & Medicine·María Rodríguez-SerranoLuisa M Sandalio
Feb 18, 2009·Plant, Cell & Environment·Antonio Jesús SerratoMariam Sahrawy
Jul 21, 2004·Journal of Ethnopharmacology·Ioannis VouldoukisBernard Dugas
Oct 15, 2009·Molecular Plant·Juan Manuel Pérez-RuizFrancisco Javier Cejudo
Oct 13, 2006·Biochimica Et Biophysica Acta·Yvonne Nyathi, Alison Baker
Jun 4, 2019·Plant, Cell & Environment·Nieves Calero-MuñozLuisa M Sandalio
Jan 8, 2020·Cells·Omar López-VidalAna Jiménez
Jun 10, 2018·BMC Plant Biology·Adoración ZafraJuan de Dios Alché

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