Immunocytochemical localization of corticosteroid-binding globulin (CBG) in guinea pig hepatocytes

Endocrinology
M Perrot-ApplanatK L David-Ferreira

Abstract

The nature of corticosteroid-binding globulin (CBG)-producing cells was studied in guinea pig liver by immunoperoxidase cytochemistry in light and electron microscopies. In light microscopy, CBG was detected in the cytoplasm of hepatocytes from pregnant and nonpregnant guinea pigs. The CBG-stained hepatocytes were more numerous in the peripheral regions of the lobules and around the portal space. In electron microscopy, CBG was associated with the hepatocyte rough endoplasmic reticulum cisternae. Patches of dense deposits were occasionally seen in the perinuclear cisternae and in cisternae identified as part of the Golgi apparatus. No deposits were seen in the smooth endoplasmic reticulum or any other cell organelles. Kupffer and bile duct cells proved to be negative to CBG. These findings demonstrate that the rough endoplasmic reticulum is the site of CBG synthesis in the guinea pig hepatocyte and confirm the hepatic origin of CBG, previously shown by biochemical methods. The distribution of CBG was also studied by light microscopy in other tissues from pregnant guinea pigs. No CBG was detected inside cells from muscle, heart, lung, kidney, ovary, uterus, or placenta. CBG was only detected in vascularized zones (glomeruli in t...Continue Reading

Citations

Mar 1, 1991·The Journal of Membrane Biology·M TruebaJ M Macarulla
Apr 1, 1990·Journal of Steroid Biochemistry·O A Strel'chyonok, G V Avvakumov
Jun 30, 1988·Biochemical and Biophysical Research Communications·A M NakhlaW Rosner
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Z S Krozowski, J W Funder
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D J HrybW Rosner
Sep 30, 2009·Molecular and Cellular Endocrinology·Jessica L Malisch, Creagh W Breuner
Apr 16, 1985·Biochemical and Biophysical Research Communications·D J HrybW Rosner
Jan 1, 1988·Annals of the New York Academy of Sciences·R W Kuhn
Jan 1, 1988·Annals of the New York Academy of Sciences·W Heyns, J L Coolens

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.