PMID: 6113879Aug 3, 1981Paper

Immunocytochemical localization of gamma-glutamyl transpeptidase in the rat CNS

Brain Research
H D Shine, B Haber

Abstract

In order to determine the cellular localization of gamma-glutamyl transpeptidase (gamma-GTP) in the nervous system the enzyme was purified to homogeneity and used to prepare an antiserum in rabbits. The anti-gamma-GTP serum cross-reacted with rodent tissues, but not those of human, monkey, calf, sheep or chicken. Monospecificity of the antibody was demonstrated by immunoelectrophoresis. The antibody inactivated the purified enzyme in a dose-dependent manner. The gamma-GTP antibody was used with the indirect fluorescent method to visualize sites containing gamma-GTP in the CNS. Choroid plexus, capillaries and the luminal side of ependymal cells stained well. Additionally, immunoreactive cells were found in both grey and white matter throughout the CNS which did not have the morphological appearance of neurons and were thought to be glia. These immunocytochemical studies suggest that gamma-GTP is largely though not exclusively associated with glia in the CNS.

References

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Citations

Jun 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H FukushimaM Ide
Jan 1, 1985·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·F SťastnýB Hájková
Jun 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B JankáskováF Stastný
Jul 6, 2000·Progress in Neurobiology·R Dringen
Aug 15, 1989·European Journal of Biochemistry·A PapandrikopoulouH G Gassen
Dec 5, 1991·European Journal of Biochemistry·A FreyH G Gassen
Oct 1, 1985·Neurochemical Research·J VeselýM Cernoch
May 1, 1983·Medical Hypotheses·F E Barr
Jan 1, 1992·Critical Reviews in Toxicology·T J Monks, S S Lau
Jan 1, 1989·Annals of the New York Academy of Sciences·J A LindgrenH E Claesson
Jan 27, 2004·Journal of Neuroscience Research·Cornelia Ruedig, Ralf Dringen
Nov 23, 2005·Journal of Neuroscience Research·Asmita DasguptaPranab Kumar Sarkar
May 9, 1997·The Journal of Biological Chemistry·B Z CarterM W Lieberman
Apr 11, 2008·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Gail D ZeevalkLaura P Bernard

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