Immunocytochemical localization of metabotropic glutamate receptor type 1 alpha and tubulin in rat brain

Neuroreport
R Lujan, Francisco Ciruela

Abstract

The distribution of mGlu1 alpha receptor and tubulin was immunocytochemically examined in the rat cerebellar cortex and primary rat cortical neurons at both immunofluorescence and electron microscopic level. In cryosections from rat cerebellar cortex mGlu1 alpha receptor immunoreactivity was expressed in cell bodies and dendrites of Purkinje and basket cells of the cerebellar molecular layer. Tubulin immunoreactivity was concentrated in the dendritic tree of the cerebellar molecular layer, as well as in the granule cell layer. In primary rat cortical neurons, both proteins colocalized throughout the proximal and distal dendrites of these cells. At the electron microscopic level, the receptor was present in dendritic shafts and dendritic spines of Purkinje cells at perisynaptic sites of asymmetrical synapses. Immunoreactivity corresponding to tubulin was associated with the plasma membrane of dendritic shafts of Purkinje cells, as well as throughout its cytoplasm as part of the cytoskeletal components. Interestingly, double labeling for both proteins reveals an association of tubulin with mGlu1 alpha receptor at the plasma membrane level of dendritic shafts of Purkinje cells. This suggests that tubulin interacts with mGlu1 alpha...Continue Reading

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Citations

Jun 25, 2009·Journal of Neural Transmission·Wacław KolasiewiczKrystyna Ossowska
Sep 18, 2010·The European Journal of Neuroscience·Francisco CiruelaRafael Luján
Jul 17, 2018·Human Molecular Genetics·Xavier MoratóFrancisco Ciruela
Nov 16, 2014·Disease Models & Mechanisms·Víctor Fernández-DueñasFrancisco Ciruela
May 6, 2004·Seminars in Cell & Developmental Biology·Laurent FagniJoel Bockaert

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