PMID: 7544404May 1, 1995Paper

Immunocytochemical localization of opsin in rod photoreceptors during periods of rapid disc assembly

Journal of Neurocytology
J C Besharse, M G Wetzel

Abstract

Transport of opsin from photoreceptor inner to outer segments has been assumed to occur via the connecting cilium, the only permanent structural connection between these two regions. However, in prior work, little or no immunoreactive opsin has been detected in the cilium, despite the high rate of transport of this protein. This suggests that immune epitopes are masked during passage through the cilium or that opsin is transported via an extra-ciliary route. In this study, we stained the photoreceptors of Xenopus laevis with well-characterized monoclonal antibodies directed at the N-terminal, C-terminal, and 5-6 loop regions of bovine opsin. This was done on isolated retinas incubated in vitro under conditions that support rapid disc assembly, to insure that opsin transport to forming discs was occurring at the time of fixation. Five MAbs that gave robust staining of Xenopus rod inner segment/rod outer segment preparations with the light microscope were utilized for electron microscopic studies on LR White embedded or cryo-ultrathin sections. Four of these stained outer segment discs and inner segment vesicles and plasma membrane. However, no significant staining of the connecting cilium was found. Furthermore, freeze-fractured...Continue Reading

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Citations

Jul 1, 1996·Histochemistry and Cell Biology·M R Torrisi, P Mancini
Feb 11, 2014·Case Reports in Medicine·B GhostineI Ghanem
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·T LiT P Dryja
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Aug 27, 2011·Experimental Eye Research·Gregory H GrossmanStephanie A Hagstrom
Jun 1, 1997·Experimental Eye Research·V MuresanJ C Besharse
Jul 13, 2000·Cell Motility and the Cytoskeleton·U Wolfrum, A Schmitt
Mar 25, 1998·FEBS Letters·B E KnoxJ C Besharse
Apr 13, 2019·Journal of Cell Science·Abhishek ChadhaDavid S Williams

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